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6bbf

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The CRAC channel Orai in an open conformation; H206A gain-of-function mutation

Structural highlights

6bbf is a 24 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 6.706Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CRCM1_DROME Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.^[1] ^[2]

Publication Abstract from PubMed

The store-operated calcium (Ca(2+)) channel Orai governs Ca(2+) influx through the plasma membrane of many non-excitable cells in metazoans. The channel opens in response to the depletion of Ca(2+) stored in the endoplasmic reticulum (ER). Loss- and gain-of-function mutants of Orai cause disease. Our previous work revealed the structure of Orai with a closed pore. Here, using a gain-of-function mutation that constitutively activates the channel, we present an X-ray structure of Drosophila melanogaster Orai in an open conformation. Well-defined electron density maps reveal that the pore is dramatically dilated on its cytosolic side in comparison to the slender closed pore. Cations and anions bind in different regions of the open pore, informing mechanisms for ion permeation and Ca(2+) selectivity. Opening of the pore requires the release of cytosolic latches. Together with additional X-ray structures of an unlatched-but-closed conformation, we propose a sequence for store-operated activation.

Structures reveal opening of the store-operated calcium channel Orai.,Hou X, Burstein SR, Long SB Elife. 2018 Aug 30;7. pii: 36758. doi: 10.7554/eLife.36758. PMID:30160233^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Feske S, Gwack Y, Prakriya M, Srikanth S, Puppel SH, Tanasa B, Hogan PG, Lewis RS, Daly M, Rao A. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature. 2006 May 11;441(7090):179-85. Epub 2006 Apr 2. PMID:16582901 doi:http://dx.doi.org/nature04702
↑ Vig M, Peinelt C, Beck A, Koomoa DL, Rabah D, Koblan-Huberson M, Kraft S, Turner H, Fleig A, Penner R, Kinet JP. CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. Science. 2006 May 26;312(5777):1220-3. Epub 2006 Apr 27. PMID:16645049 doi:http://dx.doi.org/10.1126/science.1127883
↑ Hou X, Burstein SR, Long SB. Structures reveal opening of the store-operated calcium channel Orai. Elife. 2018 Aug 30;7. pii: 36758. doi: 10.7554/eLife.36758. PMID:30160233 doi:http://dx.doi.org/10.7554/eLife.36758

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6bbf"

Categories: Drosophila melanogaster | Large Structures | Burstein S | Hou X | Long SB

@@ Line 1: / Line 1: @@
 ==The CRAC channel Orai in an open conformation; H206A gain-of-function mutation==
-<StructureSection load='6bbf' size='340' side='right' caption='[[6bbf]], [[Resolution|resolution]] 6.71&Aring;' scene=''>
+<StructureSection load='6bbf' size='340' side='right'caption='[[6bbf]], [[Resolution|resolution]] 6.71&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6bbf]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BBF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BBF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6bbf]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BBF FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hkr|4hkr]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 6.706&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bbf OCA], [http://pdbe.org/6bbf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bbf RCSB], [http://www.ebi.ac.uk/pdbsum/6bbf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bbf ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bbf OCA], [https://pdbe.org/6bbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bbf RCSB], [https://www.ebi.ac.uk/pdbsum/6bbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bbf ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CRCM1_DROME CRCM1_DROME]] Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.<ref>PMID:16582901</ref> <ref>PMID:16645049</ref>
+[https://www.uniprot.org/uniprot/CRCM1_DROME CRCM1_DROME] Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.<ref>PMID:16582901</ref> <ref>PMID:16645049</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Burstein, S]]
+[[Category: Drosophila melanogaster]]
-[[Category: Hou, X]]
+[[Category: Large Structures]]
-[[Category: Long, S B]]
+[[Category: Burstein S]]
-[[Category: Calcium]]
+[[Category: Hou X]]
-[[Category: Crac]]
+[[Category: Long SB]]
-[[Category: Eukaryotic]]
-[[Category: Ion channel]]
-[[Category: Membrane protein]]
-[[Category: Open structure]]
-[[Category: Soce]]

6bbf

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Current revision

The CRAC channel Orai in an open conformation; H206A gain-of-function mutation

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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