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| | ==Kinesin 16B Phox-homology domain (KIF16B)== | | ==Kinesin 16B Phox-homology domain (KIF16B)== |
| - | <StructureSection load='2v14' size='340' side='right' caption='[[2v14]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='2v14' size='340' side='right'caption='[[2v14]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2v14]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V14 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V14 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2v14]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V14 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v14 OCA], [http://pdbe.org/2v14 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v14 RCSB], [http://www.ebi.ac.uk/pdbsum/2v14 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v14 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v14 OCA], [https://pdbe.org/2v14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v14 RCSB], [https://www.ebi.ac.uk/pdbsum/2v14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v14 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/KI16B_HUMAN KI16B_HUMAN]] Plus end-directed microtubule-dependent motor protein involved in endosome transport and receptor recycling and degradation. Regulates the plus end motility of early endosomes and the balance between recycling and degradation of receptors such as EGF receptor (EGFR) and FGF receptor (FGFR). Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development.<ref>PMID:15882625</ref> | + | [https://www.uniprot.org/uniprot/KI16B_HUMAN KI16B_HUMAN] Plus end-directed microtubule-dependent motor protein involved in endosome transport and receptor recycling and degradation. Regulates the plus end motility of early endosomes and the balance between recycling and degradation of receptors such as EGF receptor (EGFR) and FGF receptor (FGFR). Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development.<ref>PMID:15882625</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v1/2v14_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v1/2v14_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | ==See Also== | | ==See Also== |
| - | *[[Kinesin|Kinesin]] | + | *[[Kinesin 3D Structures|Kinesin 3D Structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Blatner, N R]] | + | [[Category: Large Structures]] |
| - | [[Category: Cho, W]] | + | [[Category: Blatner NR]] |
| - | [[Category: Hong, W]] | + | [[Category: Cho W]] |
| - | [[Category: Williams, R L]] | + | [[Category: Hong W]] |
| - | [[Category: Wilson, M I]] | + | [[Category: Williams RL]] |
| - | [[Category: Alternative splicing]] | + | [[Category: Wilson MI]] |
| - | [[Category: Atp-binding]]
| + | |
| - | [[Category: Coiled coil]]
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| - | [[Category: Endosome transport]]
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| - | [[Category: Microtubule]]
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| - | [[Category: Motor protein]]
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| - | [[Category: Nucleotide-binding]]
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| - | [[Category: Phosphatidylinositol 3-phosphate binding]]
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| - | [[Category: Plus-end kinesin complex]]
| + | |
| - | [[Category: Plus-end-directed microtubule motor activity]]
| + | |
| - | [[Category: Polymorphism]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| - | [[Category: Ubl conjugation]]
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| Structural highlights
Function
KI16B_HUMAN Plus end-directed microtubule-dependent motor protein involved in endosome transport and receptor recycling and degradation. Regulates the plus end motility of early endosomes and the balance between recycling and degradation of receptors such as EGF receptor (EGFR) and FGF receptor (FGFR). Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
KIF16B is a newly identified kinesin that regulates the intracellular motility of early endosomes. KIF16B is unique among kinesins in that its cargo binding is mediated primarily by the strong interaction of its PX domain with endosomal lipids. To elucidate the structural basis of this unique endosomal anchoring activity of KIF16B-PX, we determined the crystal structure of the PX domain and performed in vitro and cellular membrane binding measurements for KIF16B-PX and mutants. The most salient structural feature of KIF16B-PX is that two neighboring residues, L1248 and F1249, on the membrane-binding surface form a protruding hydrophobic stalk with a large solvent-accessible surface area. This unique structure, arising from the complementary stacking of the two side chains and the local conformation, allows strong hydrophobic membrane interactions and endosome tethering. The presence of similar hydrophobic pairs in the amino-acid sequences of other membrane-binding domains and proteins suggests that the same structural motif may be shared by other membrane-binding proteins, whose physiological functions depend on strong hydrophobic membrane interactions.
The structural basis of novel endosome anchoring activity of KIF16B kinesin.,Blatner NR, Wilson MI, Lei C, Hong W, Murray D, Williams RL, Cho W EMBO J. 2007 Aug 8;26(15):3709-19. Epub 2007 Jul 19. PMID:17641687[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hoepfner S, Severin F, Cabezas A, Habermann B, Runge A, Gillooly D, Stenmark H, Zerial M. Modulation of receptor recycling and degradation by the endosomal kinesin KIF16B. Cell. 2005 May 6;121(3):437-50. PMID:15882625 doi:http://dx.doi.org/10.1016/j.cell.2005.02.017
- ↑ Blatner NR, Wilson MI, Lei C, Hong W, Murray D, Williams RL, Cho W. The structural basis of novel endosome anchoring activity of KIF16B kinesin. EMBO J. 2007 Aug 8;26(15):3709-19. Epub 2007 Jul 19. PMID:17641687
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