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| | ==Solution structure of the silkworm bGRP/GNBP3 N-terminal domain reveals the mechanism for b-1,3-glucan specific recognition== | | ==Solution structure of the silkworm bGRP/GNBP3 N-terminal domain reveals the mechanism for b-1,3-glucan specific recognition== |
| - | <StructureSection load='2rqe' size='340' side='right' caption='[[2rqe]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2rqe' size='340' side='right'caption='[[2rqe]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2rqe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bommo Bommo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RQE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2rqe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RQE FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqe OCA], [http://pdbe.org/2rqe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rqe RCSB], [http://www.ebi.ac.uk/pdbsum/2rqe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rqe ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqe OCA], [https://pdbe.org/2rqe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rqe RCSB], [https://www.ebi.ac.uk/pdbsum/2rqe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rqe ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BGBP_BOMMO BGBP_BOMMO]] Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade.<ref>PMID:10671539</ref> <ref>PMID:3136171</ref> | + | [https://www.uniprot.org/uniprot/BGBP_BOMMO BGBP_BOMMO] Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade.<ref>PMID:10671539</ref> <ref>PMID:3136171</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bommo]] | + | [[Category: Bombyx mori]] |
| - | [[Category: Ashida, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Horiuchi, M]] | + | [[Category: Ashida M]] |
| - | [[Category: Inagaki, F]] | + | [[Category: Horiuchi M]] |
| - | [[Category: Kumeta, H]] | + | [[Category: Inagaki F]] |
| - | [[Category: Ochiai, M]] | + | [[Category: Kumeta H]] |
| - | [[Category: Ogura, K]] | + | [[Category: Ochiai M]] |
| - | [[Category: Takahasi, K]] | + | [[Category: Ogura K]] |
| - | [[Category: 3-glucan]]
| + | [[Category: Takahasi K]] |
| - | [[Category: Beta-1]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Immune response]]
| + | |
| - | [[Category: Innate immunity]]
| + | |
| - | [[Category: Protein]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
BGBP_BOMMO Involved in the recognition of invading micro-organisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The beta-1,3-glucan recognition protein (betaGRP)/Gram-negative bacteria-binding protein 3 (GNBP3) is a crucial pattern-recognition receptor that specifically binds beta-1,3-glucan, a component of fungal cell walls. It evokes innate immunity against fungi through activation of the prophenoloxidase (proPO) cascade and Toll pathway in invertebrates. The betaGRP consists of an N-terminal beta-1,3-glucan-recognition domain and a C-terminal glucanase-like domain, with the former reported to be responsible for the proPO cascade activation. This report shows the solution structure of the N-terminal beta-1,3-glucan recognition domain of silkworm betaGRP. Although the N-terminal domain of betaGRP has a beta-sandwich fold, often seen in carbohydrate-binding modules, both NMR titration experiments and mutational analysis showed that betaGRP has a binding mechanism which is distinct from those observed in previously reported carbohydarate-binding domains. Our results suggest that betaGRP is a beta-1,3-glucan-recognition protein that specifically recognizes a triple-helical structure of beta-1,3-glucan.
Solution structure of the silkworm betaGRP/GNBP3 N-terminal domain reveals the mechanism for beta-1,3-glucan-specific recognition.,Takahasi K, Ochiai M, Horiuchi M, Kumeta H, Ogura K, Ashida M, Inagaki F Proc Natl Acad Sci U S A. 2009 Jul 14;106(28):11679-84. Epub 2009 Jun 26. PMID:19561300[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ochiai M, Ashida M. A pattern-recognition protein for beta-1,3-glucan. The binding domain and the cDNA cloning of beta-1,3-glucan recognition protein from the silkworm, Bombyx mori. J Biol Chem. 2000 Feb 18;275(7):4995-5002. PMID:10671539
- ↑ Ochiai M, Ashida M. Purification of a beta-1,3-glucan recognition protein in the prophenoloxidase activating system from hemolymph of the silkworm, Bombyx mori. J Biol Chem. 1988 Aug 25;263(24):12056-62. PMID:3136171
- ↑ Takahasi K, Ochiai M, Horiuchi M, Kumeta H, Ogura K, Ashida M, Inagaki F. Solution structure of the silkworm betaGRP/GNBP3 N-terminal domain reveals the mechanism for beta-1,3-glucan-specific recognition. Proc Natl Acad Sci U S A. 2009 Jul 14;106(28):11679-84. Epub 2009 Jun 26. PMID:19561300
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