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| | ==Structure of Corticosteroid-Binding Globulin in complex with Cortisol== | | ==Structure of Corticosteroid-Binding Globulin in complex with Cortisol== |
| - | <StructureSection load='2v95' size='340' side='right' caption='[[2v95]], [[Resolution|resolution]] 1.93Å' scene=''> | + | <StructureSection load='2v95' size='340' side='right'caption='[[2v95]], [[Resolution|resolution]] 1.93Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2v95]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2v6d 2v6d]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V95 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V95 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2v95]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2v6d 2v6d]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V95 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HCY:(11ALPHA,14BETA)-11,17,21-TRIHYDROXYPREGN-4-ENE-3,20-DIONE'>HCY</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v95 OCA], [http://pdbe.org/2v95 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v95 RCSB], [http://www.ebi.ac.uk/pdbsum/2v95 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v95 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HCY:(11ALPHA,14BETA)-11,17,21-TRIHYDROXYPREGN-4-ENE-3,20-DIONE'>HCY</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v95 OCA], [https://pdbe.org/2v95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v95 RCSB], [https://www.ebi.ac.uk/pdbsum/2v95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v95 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CBG_RAT CBG_RAT] Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.<ref>PMID:17644521</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Buffalo rat]] | + | [[Category: Large Structures]] |
| - | [[Category: Klieber, M A]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Muller, Y A]] | + | [[Category: Klieber MA]] |
| - | [[Category: Cbg]] | + | [[Category: Muller YA]] |
| - | [[Category: Corticosteroid-binding globulin transport protein]]
| + | |
| - | [[Category: Glucocorticoid]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Lipid-binding]]
| + | |
| - | [[Category: Rcl]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Serpin]]
| + | |
| - | [[Category: Steroid transporter]]
| + | |
| - | [[Category: Steroid-binding]]
| + | |
| - | [[Category: Transport]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
CBG_RAT Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Corticosteroid-binding globulin (CBG) is a serine proteinase inhibitor (serpin) family member that transports glucocorticoids in blood and regulates their access to target cells. The 1.9A crystal structure of rat CBG shows that its steroid-binding site resembles the thyroxin-binding site in the related serpin, thyroxin-binding globulin, and mutagenesis studies have confirmed the contributions of key residues that constitute the steroid-binding pocket. Unlike thyroxin-bound thyroxin-binding globulin, the cortisol-bound CBG displays an "active" serpin conformation with the proteinase-sensitive, reactive center loop (RCL) fully expelled from the regulatory beta-sheet A. Moreover, the CBG structure allows us to predict that complete insertion of the proteolytically cleaved RCL into the serpin fold occurs in concert with a displacement and unwinding of helix D that would disrupt the steroid-binding site. This allosteric coupling between RCL positioning and occupancy of the CBG steroid-binding site, which resembles the ligand (glycosamino-glycan)-dependent activation of the thrombin inhibitory serpins heparin cofactor II and anti-thrombin RCLs, ensures both optimal recognition of CBG by target proteinases and efficient release of steroid to sites of action.
Corticosteroid-binding globulin, a structural basis for steroid transport and proteinase-triggered release.,Klieber MA, Underhill C, Hammond GL, Muller YA J Biol Chem. 2007 Oct 5;282(40):29594-603. Epub 2007 Jul 19. PMID:17644521[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Klieber MA, Underhill C, Hammond GL, Muller YA. Corticosteroid-binding globulin, a structural basis for steroid transport and proteinase-triggered release. J Biol Chem. 2007 Oct 5;282(40):29594-603. Epub 2007 Jul 19. PMID:17644521 doi:10.1074/jbc.M705014200
- ↑ Klieber MA, Underhill C, Hammond GL, Muller YA. Corticosteroid-binding globulin, a structural basis for steroid transport and proteinase-triggered release. J Biol Chem. 2007 Oct 5;282(40):29594-603. Epub 2007 Jul 19. PMID:17644521 doi:10.1074/jbc.M705014200
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