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| | ==Crystal structure of ML2640c from Mycobacterium leprae in an hexagonal crystal form== | | ==Crystal structure of ML2640c from Mycobacterium leprae in an hexagonal crystal form== |
| - | <StructureSection load='2uyo' size='340' side='right' caption='[[2uyo]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='2uyo' size='340' side='right'caption='[[2uyo]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2uyo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_leprae"_hansen_1880 "bacillus leprae" hansen 1880]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UYO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UYO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2uyo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_leprae Mycobacterium leprae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UYO FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ckd|2ckd]], [[2uyq|2uyq]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uyo OCA], [http://pdbe.org/2uyo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uyo RCSB], [http://www.ebi.ac.uk/pdbsum/2uyo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2uyo ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uyo OCA], [https://pdbe.org/2uyo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uyo RCSB], [https://www.ebi.ac.uk/pdbsum/2uyo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uyo ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/Y2640_MYCLE Y2640_MYCLE]] Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.<ref>PMID:17660248</ref> | + | [https://www.uniprot.org/uniprot/Y2640_MYCLE Y2640_MYCLE] Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.<ref>PMID:17660248</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus leprae hansen 1880]] | + | [[Category: Large Structures]] |
| - | [[Category: Alzari, P M]] | + | [[Category: Mycobacterium leprae]] |
| - | [[Category: Buschiazzo, A]] | + | [[Category: Alzari PM]] |
| - | [[Category: Grana, M]] | + | [[Category: Buschiazzo A]] |
| - | [[Category: Haouz, A]] | + | [[Category: Grana M]] |
| - | [[Category: Miras, I]] | + | [[Category: Haouz A]] |
| - | [[Category: Shepard, W]] | + | [[Category: Miras I]] |
| - | [[Category: Wehenkel, A]] | + | [[Category: Shepard W]] |
| - | [[Category: Hypothetical protein]]
| + | [[Category: Wehenkel A]] |
| - | [[Category: Putative methyltransferase]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
Y2640_MYCLE Exhibits S-adenosyl-L-methionine-dependent methyltransferase activity.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Mycobacterium leprae protein ML2640c belongs to a large family of conserved hypothetical proteins predominantly found in mycobacteria, some of them predicted as putative S-adenosylmethionine (AdoMet)-dependent methyltransferases (MTase). As part of a Structural Genomics initiative on conserved hypothetical proteins in pathogenic mycobacteria, we have determined the structure of ML2640c in two distinct crystal forms. As expected, ML2640c has a typical MTase core domain and binds the methyl donor substrate AdoMet in a manner consistent with other known members of this structural family. The putative acceptor substrate-binding site of ML2640c is a large internal cavity, mostly lined by aromatic and aliphatic side-chain residues, suggesting that a lipid-like molecule might be targeted for catalysis. A flap segment (residues 222-256), which isolates the binding site from the bulk solvent and is highly mobile in the crystal structures, could serve as a gateway to allow substrate entry and product release. The multiple sequence alignment of ML2640c-like proteins revealed that the central alpha/beta core and the AdoMet-binding site are very well conserved within the family. However, the amino acid positions defining the binding site for the acceptor substrate display a higher variability, suggestive of distinct acceptor substrate specificities. The ML2640c crystal structures offer the first structural glimpses at this important family of mycobacterial proteins and lend strong support to their functional assignment as AdoMet-dependent methyltransferases.
The crystal structure of M. leprae ML2640c defines a large family of putative S-adenosylmethionine-dependent methyltransferases in mycobacteria.,Grana M, Haouz A, Buschiazzo A, Miras I, Wehenkel A, Bondet V, Shepard W, Schaeffer F, Cole ST, Alzari PM Protein Sci. 2007 Sep;16(9):1896-904. Epub 2007 Jul 27. PMID:17660248[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Grana M, Haouz A, Buschiazzo A, Miras I, Wehenkel A, Bondet V, Shepard W, Schaeffer F, Cole ST, Alzari PM. The crystal structure of M. leprae ML2640c defines a large family of putative S-adenosylmethionine-dependent methyltransferases in mycobacteria. Protein Sci. 2007 Sep;16(9):1896-904. Epub 2007 Jul 27. PMID:17660248 doi:10.1110/ps.072982707
- ↑ Grana M, Haouz A, Buschiazzo A, Miras I, Wehenkel A, Bondet V, Shepard W, Schaeffer F, Cole ST, Alzari PM. The crystal structure of M. leprae ML2640c defines a large family of putative S-adenosylmethionine-dependent methyltransferases in mycobacteria. Protein Sci. 2007 Sep;16(9):1896-904. Epub 2007 Jul 27. PMID:17660248 doi:10.1110/ps.072982707
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