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| - | {{Large structure}}
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| | ==Solution Structure of the thiolation-thioesterase di-domain of enterobactin synthetase component F== | | ==Solution Structure of the thiolation-thioesterase di-domain of enterobactin synthetase component F== |
| - | <StructureSection load='2roq' size='340' side='right' caption='[[2roq]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2roq' size='340' side='right'caption='[[2roq]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2roq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ROQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2roq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ROQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ROQ FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ent ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2roq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2roq OCA], [http://pdbe.org/2roq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2roq RCSB], [http://www.ebi.ac.uk/pdbsum/2roq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2roq ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2roq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2roq OCA], [https://pdbe.org/2roq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2roq RCSB], [https://www.ebi.ac.uk/pdbsum/2roq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2roq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | {{Large structure}} | |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ENTF_ECOLI ENTF_ECOLI]] Activates the carboxylate group of L-serine via ATP-dependent PPi exchange reactions to the aminoacyladenylate, preparing that molecule for the final stages of enterobactin synthesis. Holo-EntF acts as the catalyst for the formation of the three amide and three ester bonds present in the cyclic (2,3-dihydroxybenzoyl)serine trimer enterobactin, using seryladenylate and acyl-holo-EntB (acylated with 2,3-dihydroxybenzoate by EntE). | + | [https://www.uniprot.org/uniprot/ENTF_ECOLI ENTF_ECOLI] Activates the carboxylate group of L-serine via ATP-dependent PPi exchange reactions to the aminoacyladenylate, preparing that molecule for the final stages of enterobactin synthesis. Holo-EntF acts as the catalyst for the formation of the three amide and three ester bonds present in the cyclic (2,3-dihydroxybenzoyl)serine trimer enterobactin, using seryladenylate and acyl-holo-EntB (acylated with 2,3-dihydroxybenzoate by EntE). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| - | [[Category: Arthanari, H]] | + | [[Category: Large Structures]] |
| - | [[Category: Bennett, A E]] | + | [[Category: Arthanari H]] |
| - | [[Category: Frueh, D P]] | + | [[Category: Bennett AE]] |
| - | [[Category: Koglin, A]] | + | [[Category: Frueh DP]] |
| - | [[Category: Vosburg, D A]] | + | [[Category: Koglin A]] |
| - | [[Category: Wagner, G]] | + | [[Category: Vosburg DA]] |
| - | [[Category: Walsh, C T]] | + | [[Category: Wagner G]] |
| - | [[Category: Alpha/beta-hydrolase]]
| + | [[Category: Walsh CT]] |
| - | [[Category: Didomain]]
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| - | [[Category: Enterobactin]]
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| - | [[Category: Enterobactin biosynthesis]]
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| - | [[Category: Entf]]
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| - | [[Category: Ion transport]]
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| - | [[Category: Iron]]
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| - | [[Category: Iron transport]]
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| - | [[Category: Ligase]]
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| - | [[Category: Multifunctional enzyme]]
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| - | [[Category: Non-ribosomal peptide synthetase]]
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| - | [[Category: Nrp]]
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| - | [[Category: Pcp]]
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| - | [[Category: Peptidyl carrier protein]]
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| - | [[Category: Phosphopantetheine]]
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| - | [[Category: T-te]]
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| - | [[Category: Thioesterase]]
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| - | [[Category: Thiolation domain]]
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| - | [[Category: Transferase]]
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| - | [[Category: Transport]]
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| Structural highlights
Function
ENTF_ECOLI Activates the carboxylate group of L-serine via ATP-dependent PPi exchange reactions to the aminoacyladenylate, preparing that molecule for the final stages of enterobactin synthesis. Holo-EntF acts as the catalyst for the formation of the three amide and three ester bonds present in the cyclic (2,3-dihydroxybenzoyl)serine trimer enterobactin, using seryladenylate and acyl-holo-EntB (acylated with 2,3-dihydroxybenzoate by EntE).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Non-ribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) produce numerous secondary metabolites with various therapeutic/antibiotic properties. Like fatty acid synthases (FAS), these enzymes are organized in modular assembly lines in which each module, made of conserved domains, incorporates a given monomer unit into the growing chain. Knowledge about domain or module interactions may enable reengineering of this assembly line enzymatic organization and open avenues for the design of new bioactive compounds with improved therapeutic properties. So far, little structural information has been available on how the domains interact and communicate. This may be because of inherent interdomain mobility hindering crystallization, or because crystallized molecules may not represent the active domain orientations. In solution, the large size and internal dynamics of multidomain fragments (>35 kilodaltons) make structure determination by nuclear magnetic resonance a challenge and require advanced technologies. Here we present the solution structure of the apo-thiolation-thioesterase (T-TE) di-domain fragment of the Escherichia coli enterobactin synthetase EntF NRPS subunit. In the holoenzyme, the T domain carries the growing chain tethered to a 4'-phosphopantetheine whereas the TE domain catalyses hydrolysis and cyclization of the iron chelator enterobactin. The T-TE di-domain forms a compact but dynamic structure with a well-defined domain interface; the two active sites are at a suitable distance for substrate transfer from T to TE. We observe extensive interdomain and intradomain motions for well-defined regions and show that these are modulated by interactions with proteins that participate in the biosynthesis. The T-TE interaction described here provides a model for NRPS, PKS and FAS function in general as T-TE-like di-domains typically catalyse the last step in numerous assembly-line chain-termination machineries.
Dynamic thiolation-thioesterase structure of a non-ribosomal peptide synthetase.,Frueh DP, Arthanari H, Koglin A, Vosburg DA, Bennett AE, Walsh CT, Wagner G Nature. 2008 Aug 14;454(7206):903-6. PMID:18704088[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Frueh DP, Arthanari H, Koglin A, Vosburg DA, Bennett AE, Walsh CT, Wagner G. Dynamic thiolation-thioesterase structure of a non-ribosomal peptide synthetase. Nature. 2008 Aug 14;454(7206):903-6. PMID:18704088 doi:10.1038/nature07162
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