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| | ==THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS== | | ==THE CRYSTAL STRUCTURE OF FDXA, A 7FE FERREDOXIN FROM MYCOBACTERIUM SMEGMATIS== |
| - | <StructureSection load='2v2k' size='340' side='right' caption='[[2v2k]], [[Resolution|resolution]] 1.60Å' scene=''> | + | <StructureSection load='2v2k' size='340' side='right'caption='[[2v2k]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2v2k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V2K FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2v2k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V2K FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v2k OCA], [http://pdbe.org/2v2k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v2k RCSB], [http://www.ebi.ac.uk/pdbsum/2v2k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v2k ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v2k OCA], [https://pdbe.org/2v2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v2k RCSB], [https://www.ebi.ac.uk/pdbsum/2v2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v2k ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FER_MYCSM FER_MYCSM]] Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. | + | [https://www.uniprot.org/uniprot/FER_MYCSM FER_MYCSM] Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Ferredoxin|Ferredoxin]] | + | *[[Ferredoxin 3D structures|Ferredoxin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Mycobacterium smegmatis]] | + | [[Category: Large Structures]] |
| - | [[Category: Aliverti, A]] | + | [[Category: Mycolicibacterium smegmatis MC2 155]] |
| - | [[Category: Bolognesi, M]] | + | [[Category: Aliverti A]] |
| - | [[Category: Ricagno, S]] | + | [[Category: Bolognesi M]] |
| - | [[Category: Rosa, M de]]
| + | [[Category: Ricagno S]] |
| - | [[Category: Zanetti, G]] | + | [[Category: Zanetti G]] |
| - | [[Category: 4fe-4s cluster instability]] | + | [[Category: De Rosa M]] |
| - | [[Category: 7fe ferredoxin]]
| + | |
| - | [[Category: Electron transfer]]
| + | |
| - | [[Category: Electron transport]]
| + | |
| - | [[Category: Fe-s cluster]]
| + | |
| - | [[Category: Iron]]
| + | |
| - | [[Category: Iron-sulfur]]
| + | |
| - | [[Category: Metal-binding]]
| + | |
| - | [[Category: Mycobacterium smegmati]]
| + | |
| - | [[Category: Mycobacterium tuberculosis]]
| + | |
| - | [[Category: Transport]]
| + | |
| Structural highlights
Function
FER_MYCSM Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Mycobacterium smegmatis ferredoxin FdxA, which has an orthologue ferredoxin in Mycobacterium tuberculosis, FdxC, contains both one [3Fe-4S] and one [4Fe-4S] cluster. M. smegmatis FdxA has been shown to be a preferred ferredoxin substrate of FprA [F. Fischer, D. Raimondi, A. Aliverti, G. Zanetti, Mycobacterium tuberculosis FprA, a novel bacterial NADPH-ferredoxin reductase, Eur. J. Biochem. 269 (2002) 3005-3013], an adrenodoxin reductase-like flavoprotein of M. tuberculosis, suggesting that M. tuberculosis FdxC could be the physiological partner of the enzyme in providing reducing power to the cytochromes P450. We report here the crystal structure of FdxA at 1.6A resolution (R(factor) 16.5%, R(free) 20.2%). Besides providing an insight on protein architecture for this 106-residue ferredoxin, our crystallographic investigation highlights lability of the [4Fe-4S] center, which is shown to loose a Fe atom during crystal growth. Due to their high similarity (87% sequence identity), the structure here reported can be considered a valuable model for M. tuberculosis FdxC, thus representing a step forward in the study of the complex mycobacterial redox pathways.
The crystal structure of FdxA, a 7Fe ferredoxin from Mycobacterium smegmatis.,Ricagno S, de Rosa M, Aliverti A, Zanetti G, Bolognesi M Biochem Biophys Res Commun. 2007 Aug 17;360(1):97-102. Epub 2007 Jun 12. PMID:17577575[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ricagno S, de Rosa M, Aliverti A, Zanetti G, Bolognesi M. The crystal structure of FdxA, a 7Fe ferredoxin from Mycobacterium smegmatis. Biochem Biophys Res Commun. 2007 Aug 17;360(1):97-102. Epub 2007 Jun 12. PMID:17577575 doi:10.1016/j.bbrc.2007.06.013
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