2q13

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[[Image:2q13.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of BAR-PH domain of APPL1==
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|PDB= 2q13 |SIZE=350|CAPTION= <scene name='initialview01'>2q13</scene>, resolution 2.05&Aring;
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<StructureSection load='2q13' size='340' side='right'caption='[[2q13]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2q13]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q13 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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|GENE= APPL1, APPL, DIP13A, KIAA1428 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q13 OCA], [https://pdbe.org/2q13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q13 RCSB], [https://www.ebi.ac.uk/pdbsum/2q13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q13 ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[2q12|2Q12]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q13 OCA], [http://www.ebi.ac.uk/pdbsum/2q13 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2q13 RCSB]</span>
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[https://www.uniprot.org/uniprot/DP13A_HUMAN DP13A_HUMAN] Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.<ref>PMID:10490823</ref> <ref>PMID:15016378</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''Crystal structure of BAR-PH domain of APPL1'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q1/2q13_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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APPL1 is an effector of the small GTPase Rab5. Together, they mediate a signal transduction pathway initiated by ligand binding to cell surface receptors. Interaction with Rab5 is confined to the amino (N)-terminal region of APPL1. We report the crystal structures of human APPL1 N-terminal BAR-PH domain motif. The BAR and PH domains, together with a novel linker helix, form an integrated, crescent-shaped, symmetrical dimer. This BAR-PH interaction is likely conserved in the class of BAR-PH containing proteins. Biochemical analyses indicate two independent Rab-binding sites located at the opposite ends of the dimer, where the PH domain directly interacts with Rab5 and Rab21. Besides structurally supporting the PH domain, the BAR domain also contributes to Rab binding through a small surface region in the vicinity of the PH domain. In stark contrast to the helix-dominated, Rab-binding domains previously reported, APPL1 PH domain employs beta-strands to interact with Rab5. On the Rab5 side, both switch regions are involved in the interaction. Thus we identified a new binding mode between PH domains and small GTPases.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q13 ConSurf].
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2Q13 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q13 OCA].
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<div style="clear:both"></div>
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== References ==
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==Reference==
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<references/>
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Structure of the APPL1 BAR-PH domain and characterization of its interaction with Rab5., Zhu G, Chen J, Liu J, Brunzelle JS, Huang B, Wakeham N, Terzyan S, Li X, Rao Z, Li G, Zhang XC, EMBO J. 2007 Jul 25;26(14):3484-93. Epub 2007 Jun 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17581628 17581628]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Zhang, X C.]]
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[[Category: Zhang XC]]
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[[Category: Zhu, G.]]
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[[Category: Zhu G]]
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[[Category: appl1]]
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[[Category: bar domain]]
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[[Category: bar-ph domain]]
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[[Category: ph domain]]
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[[Category: protein transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:43:18 2008''
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Current revision

Crystal structure of BAR-PH domain of APPL1

PDB ID 2q13

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