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| | ==Crystal structure of N-terminal domains of Human La protein complexed with RNA oligomer AUUUU== | | ==Crystal structure of N-terminal domains of Human La protein complexed with RNA oligomer AUUUU== |
| - | <StructureSection load='2vop' size='340' side='right' caption='[[2vop]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='2vop' size='340' side='right'caption='[[2vop]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2vop]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VOP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VOP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2vop]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VOP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VOP FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1s7a|1s7a]], [[1zh5|1zh5]], [[1yty|1yty]], [[1s79|1s79]], [[2voo|2voo]], [[2vod|2vod]], [[2von|2von]], [[1owx|1owx]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vop OCA], [http://pdbe.org/2vop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vop RCSB], [http://www.ebi.ac.uk/pdbsum/2vop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vop ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vop OCA], [https://pdbe.org/2vop PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vop RCSB], [https://www.ebi.ac.uk/pdbsum/2vop PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vop ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/LA_HUMAN LA_HUMAN] Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.<ref>PMID:3192525</ref> <ref>PMID:2470590</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Conte, M R]] | + | [[Category: Large Structures]] |
| - | [[Category: Curry, S]] | + | [[Category: Conte MR]] |
| - | [[Category: Kotik-Kogan, O]] | + | [[Category: Curry S]] |
| - | [[Category: Sanfelice, D]] | + | [[Category: Kotik-Kogan O]] |
| - | [[Category: Valentine, E R]] | + | [[Category: Sanfelice D]] |
| - | [[Category: La motif]]
| + | [[Category: Valentine ER]] |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Phosphoprotein]]
| + | |
| - | [[Category: Polymorphism]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Rna maturation]]
| + | |
| - | [[Category: Rna recognition motif]]
| + | |
| - | [[Category: Rna-binding]]
| + | |
| - | [[Category: Rna-binding protein]]
| + | |
| - | [[Category: Systemic lupus erythematosus]]
| + | |
| Structural highlights
Function
LA_HUMAN Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The eukaryotic La protein recognizes the 3' poly(U) sequences of nascent RNA polymerase III transcripts to assist folding and maturation. The 3' ends of such RNAs are bound by the N-terminal domain of La (LaNTD). We have solved the crystal structures of four LaNTD:RNA complexes, each containing a different single-stranded RNA oligomer, and compared them to the structure of a previously published LaNTD:RNA complex containing partially duplex RNA. The presence of purely single-stranded RNA in the binding pocket at the interface between the La motif and RRM domains allows significantly closer contact with the 3' end of the RNA. Comparison of the different LaNTD:RNA complexes identifies a conserved set of interactions with the last two nucleotides at the 3' end of the RNA ligand that are key to binding. Strikingly, we also observe two alternative conformations of bound ssRNA, indicative of an unexpected degree of plasticity in the modes of RNA binding.
Structural analysis reveals conformational plasticity in the recognition of RNA 3' ends by the human La protein.,Kotik-Kogan O, Valentine ER, Sanfelice D, Conte MR, Curry S Structure. 2008 Jun;16(6):852-62. PMID:18547518[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chambers JC, Kenan D, Martin BJ, Keene JD. Genomic structure and amino acid sequence domains of the human La autoantigen. J Biol Chem. 1988 Dec 5;263(34):18043-51. PMID:3192525
- ↑ Gottlieb E, Steitz JA. Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III. EMBO J. 1989 Mar;8(3):851-61. PMID:2470590
- ↑ Kotik-Kogan O, Valentine ER, Sanfelice D, Conte MR, Curry S. Structural analysis reveals conformational plasticity in the recognition of RNA 3' ends by the human La protein. Structure. 2008 Jun;16(6):852-62. PMID:18547518 doi:http://dx.doi.org/10.1016/j.str.2008.02.021
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