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| | ==Structure of mouse A1 bound to the Bmf BH3-domain== | | ==Structure of mouse A1 bound to the Bmf BH3-domain== |
| - | <StructureSection load='2vog' size='340' side='right' caption='[[2vog]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='2vog' size='340' side='right'caption='[[2vog]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2vog]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VOG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VOG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2vog]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VOG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VOG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vof|2vof]], [[2voh|2voh]], [[2voi|2voi]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vog OCA], [http://pdbe.org/2vog PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vog RCSB], [http://www.ebi.ac.uk/pdbsum/2vog PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vog ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vog OCA], [https://pdbe.org/2vog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vog RCSB], [https://www.ebi.ac.uk/pdbsum/2vog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vog ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BMF_MOUSE BMF_MOUSE]] May play a role in apoptosis. | + | [https://www.uniprot.org/uniprot/B2LA1_MOUSE B2LA1_MOUSE] Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection. Can inhibit apoptosis induced by serum starvation in the mammary epithelial cell line HC11 (PubMed:11888890).<ref>PMID:11888890</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[B-cell lymphoma protein|B-cell lymphoma protein]] | + | *[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Czabotar, P E]] | + | [[Category: Mus musculus]] |
| - | [[Category: Day, C L]] | + | [[Category: Czabotar PE]] |
| - | [[Category: Hinds, M G]] | + | [[Category: Day CL]] |
| - | [[Category: Smits, C]] | + | [[Category: Hinds MG]] |
| - | [[Category: Apoptosis]] | + | [[Category: Smits C]] |
| - | [[Category: Bcl-2]]
| + | |
| - | [[Category: Bh3]]
| + | |
| - | [[Category: Pro-survival]]
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| - | [[Category: Protein-protein complex]]
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| Structural highlights
Function
B2LA1_MOUSE Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection. Can inhibit apoptosis induced by serum starvation in the mammary epithelial cell line HC11 (PubMed:11888890).[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Apoptotic pathways are regulated by protein-protein interactions. Interaction of the BH3 domains of proapoptotic Bcl-2 family proteins with the hydrophobic groove of prosurvival proteins is critical. Whereas some BH3 domains bind in a promiscuous manner, others exhibit considerable selectivity and the sequence characteristics that distinguish these activities are unclear. In this study, crystal structures of complexes between the prosurvival protein A1 and the BH3 domains from Puma, Bmf, Bak, and Bid have been solved. The structure of A1 is similar to that of other prosurvival proteins, although features, such as an acidic patch in the binding groove, may allow specific therapeutic modulation of apoptosis. Significant conformational plasticity was observed in the intermolecular interactions and these differences explain some of the variation in affinity. This study, in combination with published data, suggests that interactions between conserved residues demarcate optimal binding.
Structural plasticity underpins promiscuous binding of the prosurvival protein A1.,Smits C, Czabotar PE, Hinds MG, Day CL Structure. 2008 May;16(5):818-29. PMID:18462686[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ha S, Lee S, Chung M, Choi Y. Mouse ING1 homologue, a protein interacting with A1, enhances cell death and is inhibited by A1 in mammary epithelial cells. Cancer Res. 2002 Mar 1;62(5):1275-8 PMID:11888890
- ↑ Smits C, Czabotar PE, Hinds MG, Day CL. Structural plasticity underpins promiscuous binding of the prosurvival protein A1. Structure. 2008 May;16(5):818-29. PMID:18462686 doi:http://dx.doi.org/10.1016/j.str.2008.02.009
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