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2wop

From Proteopedia

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Clavulanic acid biosynthesis oligopeptide binding protein 2 complexed with arginine

Structural highlights

2wop is a 1 chain structure with sequence from Streptomyces clavuligerus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.7Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8KRB4_STRCL

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Clavulanic acid (CA) is a clinically important beta-lactamase inhibitor that is produced by fermentation of Streptomyces clavuligerus. The CA biosynthesis pathway starts from arginine and glyceraldehyde-3-phosphate and proceeds via (3S,5S)-clavaminic acid, which is converted to (3R,5R)-clavaldehyde, the immediate precursor of (3R,5R)-CA. Open reading frames 7 (orf7) and 15 (orf15) of the CA biosynthesis cluster encode oligopeptide-binding proteins (OppA1 and OppA2), which are essential for CA biosynthesis. OppA1/2 are proposed to be involved in the binding and/or transport of peptides across the S. clavuligerus cell membrane. Peptide binding assays reveal that recombinant OppA1 and OppA2 bind di-/tripeptides containing arginine and certain nonapeptides including bradykinin. Crystal structures of OppA2 in its apo form and in complex with arginine or bradykinin were solved to 1.45, 1.7, and 1.7 A resolution, respectively. The overall fold of OppA2 consists of two lobes with a deep cavity in the center, as observed for other oligopeptide-binding proteins. The large cavity creates a peptide/arginine binding cleft. The crystal structures of OppA2 in complex with arginine or bradykinin reveal that the C-terminal arginine of bradykinin binds similarly to arginine. The results are discussed in terms of the possible roles of OppA1/2 in CA biosynthesis.

Crystal structures of an oligopeptide-binding protein from the biosynthetic pathway of the beta-lactamase inhibitor clavulanic acid.,Mackenzie AK, Valegard K, Iqbal A, Caines ME, Kershaw NJ, Jensen SE, Schofield CJ, Andersson I J Mol Biol. 2010 Feb 19;396(2):332-44. Epub 2009 Nov 24. PMID:19941870^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mackenzie AK, Valegard K, Iqbal A, Caines ME, Kershaw NJ, Jensen SE, Schofield CJ, Andersson I. Crystal structures of an oligopeptide-binding protein from the biosynthetic pathway of the beta-lactamase inhibitor clavulanic acid. J Mol Biol. 2010 Feb 19;396(2):332-44. Epub 2009 Nov 24. PMID:19941870 doi:10.1016/j.jmb.2009.11.045

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wop"

@@ Line 1: / Line 1: @@
 ==Clavulanic acid biosynthesis oligopeptide binding protein 2 complexed with arginine==
-<StructureSection load='2wop' size='340' side='right' caption='[[2wop]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+<StructureSection load='2wop' size='340' side='right'caption='[[2wop]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wop]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1611 As 4.1611]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WOP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WOP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wop]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WOP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WOP FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wol|2wol]], [[2wok|2wok]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wop OCA], [http://pdbe.org/2wop PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wop RCSB], [http://www.ebi.ac.uk/pdbsum/2wop PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wop ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wop OCA], [https://pdbe.org/2wop PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wop RCSB], [https://www.ebi.ac.uk/pdbsum/2wop PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wop ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8KRB4_STRCL Q8KRB4_STRCL]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 29: / Line 31: @@
 ==See Also==
-*[[Oligopeptide-binding protein|Oligopeptide-binding protein]]
+*[[ABC transporter 3D structures|ABC transporter 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: As 4 1611]]
+[[Category: Large Structures]]
-[[Category: Andersson, I]]
+[[Category: Streptomyces clavuligerus]]
-[[Category: Caines, M E.C]]
+[[Category: Andersson I]]
-[[Category: Iqbal, A]]
+[[Category: Caines MEC]]
-[[Category: Jensen, S E]]
+[[Category: Iqbal A]]
-[[Category: Kershaw, N J]]
+[[Category: Jensen SE]]
-[[Category: MacKenzie, A K]]
+[[Category: Kershaw NJ]]
-[[Category: Schofield, C J]]
+[[Category: MacKenzie AK]]
-[[Category: Valegard, K]]
+[[Category: Schofield CJ]]
-[[Category: Clavulanic acid biosynthesis]]
+[[Category: Valegard K]]
-[[Category: Oligopeptide binding protein]]
-[[Category: Peptide binding protein]]
-[[Category: Peptide binding protein-peptide complex]]
-[[Category: Solute-binding protein]]

2wop

From Proteopedia

Current revision

Clavulanic acid biosynthesis oligopeptide binding protein 2 complexed with arginine

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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