This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2wmc

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Crystal structure of eukaryotic initiation factor 4E from Pisum sativum

Structural highlights

2wmc is a 8 chain structure with sequence from Pisum sativum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IF4E1_PEA Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed:15469495, PubMed:21283665). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (PubMed:15469495, PubMed:21283665). Key component of recessive resistance to potyviruses (PubMed:15469495, PubMed:17849710, PubMed:21283665, PubMed:24609094).^[1] ^[2] ^[3] ^[4] (Microbial infection) Susceptibility host factor required for viral infection by recruiting viral RNAs to the host ribosomal complex via an interaction with viral genome-linked protein (VPg) (PubMed:15469495, PubMed:17849710, PubMed:24609094, PubMed:21283665). Seems also involved in virus movement from cell-to-cell (PubMed:15469495).^[5] ^[6] ^[7] ^[8]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

BACKGROUND: Pea encodes eukaryotic translation initiation factor eIF4E (eIF4E(S)), which supports the multiplication of Pea seed-borne mosaic virus (PSbMV). In common with hosts for other potyviruses, some pea lines contain a recessive allele (sbm1) encoding a mutant eIF4E (eIF4E(R)) that fails to interact functionally with the PSbMV avirulence protein, VPg, giving genetic resistance to infection. METHODOLOGY/PRINCIPAL FINDINGS: To study structure-function relationships between pea eIF4E and PSbMV VPg, we obtained an X-ray structure for eIF4E(S) bound to m(7)GTP. The crystallographic asymmetric unit contained eight independent copies of the protein, providing insights into the structurally conserved and flexible regions of eIF4E. To assess indirectly the importance of key residues in binding to VPg and/or m(7)GTP, an extensive range of point mutants in eIF4E was tested for their ability to complement PSbMV multiplication in resistant pea tissues and for complementation of protein translation, and hence growth, in an eIF4E-defective yeast strain conditionally dependent upon ectopic expression of eIF4E. The mutants also dissected individual contributions from polymorphisms present in eIF4E(R) and compared the impact of individual residues altered in orthologous resistance alleles from other crop species. The data showed that essential resistance determinants in eIF4E differed for different viruses although the critical region involved (possibly in VPg-binding) was conserved and partially overlapped with the m(7)GTP-binding region. This overlap resulted in coupled inhibition of virus multiplication and translation in the majority of cases, although the existence of a few mutants that uncoupled the two processes supported the view that the specific role of eIF4E in potyvirus infection may not be restricted to translation. CONCLUSIONS/SIGNIFICANCE: The work describes the most extensive structural analysis of eIF4E in relation to potyvirus resistance. In addition to defining functional domains within the eIF4E structure, we identified eIF4E alleles with the potential to convey novel virus resistance phenotypes.

Structure-Based Mutational Analysis of eIF4E in Relation to sbm1 Resistance to Pea Seed-Borne Mosaic Virus in Pea.,Ashby JA, Stevenson CE, Jarvis GE, Lawson DM, Maule AJ PLoS One. 2011 Jan 24;6(1):e15873. PMID:21283665^[9]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gao Z, Johansen E, Eyers S, Thomas CL, Noel Ellis TH, Maule AJ. The potyvirus recessive resistance gene, sbm1, identifies a novel role for translation initiation factor eIF4E in cell-to-cell trafficking. Plant J. 2004 Nov;40(3):376-85. PMID:15469495 doi:10.1111/j.1365-313X.2004.02215.x
↑ Bruun-Rasmussen M, Møller IS, Tulinius G, Hansen JK, Lund OS, Johansen IE. The same allele of translation initiation factor 4E mediates resistance against two Potyvirus spp. in Pisum sativum. Mol Plant Microbe Interact. 2007 Sep;20(9):1075-82. PMID:17849710 doi:10.1094/MPMI-20-9-1075
↑ Ashby JA, Stevenson CE, Jarvis GE, Lawson DM, Maule AJ. Structure-Based Mutational Analysis of eIF4E in Relation to sbm1 Resistance to Pea Seed-Borne Mosaic Virus in Pea. PLoS One. 2011 Jan 24;6(1):e15873. PMID:21283665 doi:10.1371/journal.pone.0015873
↑ Konečná E, Šafářová D, Navrátil M, Hanáček P, Coyne C, Flavell A, Vishnyakova M, Ambrose M, Redden R, Smýkal P. Geographical gradient of the eIF4E alleles conferring resistance to potyviruses in pea (Pisum) germplasm. PLoS One. 2014 Mar 7;9(3):e90394. PMID:24609094 doi:10.1371/journal.pone.0090394
↑ Gao Z, Johansen E, Eyers S, Thomas CL, Noel Ellis TH, Maule AJ. The potyvirus recessive resistance gene, sbm1, identifies a novel role for translation initiation factor eIF4E in cell-to-cell trafficking. Plant J. 2004 Nov;40(3):376-85. PMID:15469495 doi:10.1111/j.1365-313X.2004.02215.x
↑ Bruun-Rasmussen M, Møller IS, Tulinius G, Hansen JK, Lund OS, Johansen IE. The same allele of translation initiation factor 4E mediates resistance against two Potyvirus spp. in Pisum sativum. Mol Plant Microbe Interact. 2007 Sep;20(9):1075-82. PMID:17849710 doi:10.1094/MPMI-20-9-1075
↑ Ashby JA, Stevenson CE, Jarvis GE, Lawson DM, Maule AJ. Structure-Based Mutational Analysis of eIF4E in Relation to sbm1 Resistance to Pea Seed-Borne Mosaic Virus in Pea. PLoS One. 2011 Jan 24;6(1):e15873. PMID:21283665 doi:10.1371/journal.pone.0015873
↑ Konečná E, Šafářová D, Navrátil M, Hanáček P, Coyne C, Flavell A, Vishnyakova M, Ambrose M, Redden R, Smýkal P. Geographical gradient of the eIF4E alleles conferring resistance to potyviruses in pea (Pisum) germplasm. PLoS One. 2014 Mar 7;9(3):e90394. PMID:24609094 doi:10.1371/journal.pone.0090394
↑ Ashby JA, Stevenson CE, Jarvis GE, Lawson DM, Maule AJ. Structure-Based Mutational Analysis of eIF4E in Relation to sbm1 Resistance to Pea Seed-Borne Mosaic Virus in Pea. PLoS One. 2011 Jan 24;6(1):e15873. PMID:21283665 doi:10.1371/journal.pone.0015873

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wmc"

@@ Line 1: / Line 1: @@
 ==Crystal structure of eukaryotic initiation factor 4E from Pisum sativum==
-<StructureSection load='2wmc' size='340' side='right' caption='[[2wmc]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='2wmc' size='340' side='right'caption='[[2wmc]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wmc]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Garden_pea Garden pea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WMC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WMC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wmc]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WMC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WMC FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MGP:7-METHYL-GUANOSINE-5-TRIPHOSPHATE'>MGP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wmc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wmc OCA], [http://pdbe.org/2wmc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wmc RCSB], [http://www.ebi.ac.uk/pdbsum/2wmc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wmc ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MGP:7-METHYL-GUANOSINE-5-TRIPHOSPHATE'>MGP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wmc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wmc OCA], [https://pdbe.org/2wmc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wmc RCSB], [https://www.ebi.ac.uk/pdbsum/2wmc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wmc ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/IF4E1_PEA IF4E1_PEA] Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed:15469495, PubMed:21283665). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (PubMed:15469495, PubMed:21283665). Key component of recessive resistance to potyviruses (PubMed:15469495, PubMed:17849710, PubMed:21283665, PubMed:24609094).<ref>PMID:15469495</ref> <ref>PMID:17849710</ref> <ref>PMID:21283665</ref> <ref>PMID:24609094</ref>   (Microbial infection) Susceptibility host factor required for viral infection by recruiting viral RNAs to the host ribosomal complex via an interaction with viral genome-linked protein (VPg) (PubMed:15469495, PubMed:17849710, PubMed:24609094, PubMed:21283665). Seems also involved in virus movement from cell-to-cell (PubMed:15469495).<ref>PMID:15469495</ref> <ref>PMID:17849710</ref> <ref>PMID:21283665</ref> <ref>PMID:24609094</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 28: / Line 31: @@
 ==See Also==
-*[[Eukaryotic initiation factor|Eukaryotic initiation factor]]
+*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Garden pea]]
+[[Category: Large Structures]]
-[[Category: Ashby, J A]]
-[[Category: Lawson, D M]]
-[[Category: Maule, A J]]
-[[Category: Stevenson, C E.M]]
-[[Category: Biosynthetic protein]]
-[[Category: Eif4e]]
-[[Category: Initiation factor]]
 [[Category: Pisum sativum]]
-[[Category: Protein biosynthesis]]
+[[Category: Ashby JA]]
-[[Category: Rna-binding]]
+[[Category: Lawson DM]]
-[[Category: Sbm1 gene]]
+[[Category: Maule AJ]]
+[[Category: Stevenson CEM]]

2wmc

From Proteopedia

Current revision

Crystal structure of eukaryotic initiation factor 4E from Pisum sativum

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox