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| - | [[Image:2qh2.gif|left|200px]] | |
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| - | {{Structure
| + | ==Solution structure of the CR7 terminal hairpin loop from human telomerase RNA== |
| - | |PDB= 2qh2 |SIZE=350|CAPTION= <scene name='initialview01'>2qh2</scene>
| + | <StructureSection load='2qh2' size='340' side='right'caption='[[2qh2]]' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | <table><tr><td colspan='2'>[[2qh2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QH2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QH2 FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | |GENE=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qh2 OCA], [https://pdbe.org/2qh2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qh2 RCSB], [https://www.ebi.ac.uk/pdbsum/2qh2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qh2 ProSAT]</span></td></tr> |
| - | |DOMAIN=
| + | </table> |
| - | |RELATEDENTRY=
| + | |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qh2 OCA], [http://www.ebi.ac.uk/pdbsum/2qh2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qh2 RCSB]</span>
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| - | }}
| + | |
| | | | |
| - | '''Solution structure of the CR7 terminal hairpin loop from human telomerase RNA'''
| + | ==See Also== |
| - | | + | *[[Telomerase 3D structures|Telomerase 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains required for 3' end processing, localization, and accumulation. The terminal loop of the CR7 domain contains the CAB box (ugAG) required for targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence required for accumulation and processing. To dissect out the contributions of the CR7 stem loop to hTR processing and localization, we solved the solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These structures, together with analysis of localization, processing, and accumulation of hTRs containing nucleotide substitutions in the CR7 domain, identified the sequence and structural requirements of the hTR processing and CB localization signals and showed that these signals are functionally independent. Further, 3' end processing was found to be a prerequisite for translocation of hTR to CBs.
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Breece KE]] |
| - | ==About this Structure==
| + | [[Category: Chim N]] |
| - | 2QH2 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QH2 OCA].
| + | [[Category: Feigon J]] |
| - | | + | [[Category: Theimer CA]] |
| - | ==Reference==
| + | |
| - | Structural and functional characterization of human telomerase RNA processing and cajal body localization signals., Theimer CA, Jady BE, Chim N, Richard P, Breece KE, Kiss T, Feigon J, Mol Cell. 2007 Sep 21;27(6):869-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17889661 17889661]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Breece, K E.]] | + | |
| - | [[Category: Chim, N]] | + | |
| - | [[Category: Feigon, J.]] | + | |
| - | [[Category: Theimer, C A.]] | + | |
| - | [[Category: loop]]
| + | |
| - | [[Category: rna]]
| + | |
| - | [[Category: syn g]]
| + | |
| - | [[Category: wobble base pair]]
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| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:49:14 2008''
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