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| | ==3D structure of YmoB, a modulator of biofilm formation== | | ==3D structure of YmoB, a modulator of biofilm formation== |
| - | <StructureSection load='2mn2' size='340' side='right' caption='[[2mn2]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2mn2' size='340' side='right'caption='[[2mn2]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mn2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_enterocoliticum"_schleifstein_and_coleman_1939 "bacterium enterocoliticum" schleifstein and coleman 1939]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MN2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MN2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mn2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MN2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MN2 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ybaJ, YEW_DG13760, ymoB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=630 "Bacterium enterocoliticum" Schleifstein and Coleman 1939])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mn2 OCA], [http://pdbe.org/2mn2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mn2 RCSB], [http://www.ebi.ac.uk/pdbsum/2mn2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mn2 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mn2 OCA], [https://pdbe.org/2mn2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mn2 RCSB], [https://www.ebi.ac.uk/pdbsum/2mn2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mn2 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0E1NKQ3_YEREN A0A0E1NKQ3_YEREN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacterium enterocoliticum schleifstein and coleman 1939]] | + | [[Category: Large Structures]] |
| - | [[Category: Amata, I]] | + | [[Category: Yersinia enterocolitica]] |
| - | [[Category: Cordeiro, T N]] | + | [[Category: Amata I]] |
| - | [[Category: Marimon, O]] | + | [[Category: Cordeiro TN]] |
| - | [[Category: Pons, M]] | + | [[Category: Marimon O]] |
| - | [[Category: Antitoxin]]
| + | [[Category: Pons M]] |
| - | [[Category: Buried cysteine]]
| + | |
| - | [[Category: Four helix bundle]]
| + | |
| Structural highlights
Function
A0A0E1NKQ3_YEREN
Publication Abstract from PubMed
The Hha and TomB proteins from Escherichia coli form an oxygen-dependent toxin-antitoxin (TA) system. Here we show that YmoB, the Yersinia orthologue of TomB, and its single cysteine variant [C117S]YmoB can replace TomB as antitoxins in E. coli. In contrast to other TA systems, [C117S]YmoB transiently interacts with Hha (rather than forming a stable complex) and enhances the spontaneous oxidation of the Hha conserved cysteine residue to a -SOxH-containing species (sulfenic, sulfinic or sulfonic acid), which destabilizes the toxin. The nuclear magnetic resonance structure of [C117S]YmoB and the homology model of TomB show that the two proteins form a four-helix bundle with a conserved buried cysteine connected to the exterior by a channel with a diameter comparable to that of an oxygen molecule. The Hha interaction site is located on the opposite side of the helix bundle.
An oxygen-sensitive toxin-antitoxin system.,Marimon O, Teixeira JM, Cordeiro TN, Soo VW, Wood TL, Mayzel M, Amata I, Garcia J, Morera A, Gay M, Vilaseca M, Orekhov VY, Wood TK, Pons M Nat Commun. 2016 Dec 8;7:13634. doi: 10.1038/ncomms13634. PMID:27929062[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Marimon O, Teixeira JM, Cordeiro TN, Soo VW, Wood TL, Mayzel M, Amata I, Garcia J, Morera A, Gay M, Vilaseca M, Orekhov VY, Wood TK, Pons M. An oxygen-sensitive toxin-antitoxin system. Nat Commun. 2016 Dec 8;7:13634. doi: 10.1038/ncomms13634. PMID:27929062 doi:http://dx.doi.org/10.1038/ncomms13634
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