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| | ==Drosophila Melanogaster (6-4) Photolyase Bound To double stranded Dna containing a T(6-4)C Photolesion== | | ==Drosophila Melanogaster (6-4) Photolyase Bound To double stranded Dna containing a T(6-4)C Photolesion== |
| - | <StructureSection load='2wb2' size='340' side='right' caption='[[2wb2]], [[Resolution|resolution]] 2.95Å' scene=''> | + | <StructureSection load='2wb2' size='340' side='right'caption='[[2wb2]], [[Resolution|resolution]] 2.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2wb2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WB2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WB2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2wb2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WB2 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=64P:5-AMINOTHYMIDINE+5-(DIHYDROGEN+PHOSPHATE)'>64P</scene>, <scene name='pdbligand=Z:1-(2-DEOXY-5-O-PHOSPHONO-BETA-D-ERYTHRO-PENTOFURANOSYL)PYRIMIDIN-2(1H)-ONE'>Z</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=64P:5-AMINOTHYMIDINE+5-(DIHYDROGEN+PHOSPHATE)'>64P</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=Z:1-(2-DEOXY-5-O-PHOSPHONO-BETA-D-ERYTHRO-PENTOFURANOSYL)PYRIMIDIN-2(1H)-ONE'>Z</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribodipyrimidine_photo-lyase Deoxyribodipyrimidine photo-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.3 4.1.99.3] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wb2 OCA], [https://pdbe.org/2wb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wb2 RCSB], [https://www.ebi.ac.uk/pdbsum/2wb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wb2 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wb2 OCA], [http://pdbe.org/2wb2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wb2 RCSB], [http://www.ebi.ac.uk/pdbsum/2wb2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wb2 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8SXK5_DROME Q8SXK5_DROME] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Deoxyribodipyrimidine photo-lyase]] | + | [[Category: Drosophila melanogaster]] |
| - | [[Category: Drome]] | + | [[Category: Large Structures]] |
| - | [[Category: Carell, T]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Glas, A F]] | + | [[Category: Carell T]] |
| - | [[Category: Hennecke, U]] | + | [[Category: Glas AF]] |
| - | [[Category: Maul, M J]] | + | [[Category: Hennecke U]] |
| - | [[Category: Schneider, S]] | + | [[Category: Maul MJ]] |
| - | [[Category: Dna photolyase]]
| + | [[Category: Schneider S]] |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Lyase-dna complex]]
| + | |
| - | [[Category: Photolesion]]
| + | |
| Structural highlights
Function
Q8SXK5_DROME
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
UV-light irradiation induces the formation of highly mutagenic lesions in DNA, such as cis-syn cyclobutane pyrimidine dimers (CPD photoproducts), pyrimidine(6-4)pyrimidone photoproducts ((6-4) photoproducts) and their Dewar valence isomers ((Dew) photoproducts). Here we describe the synthesis of defined DNA strands containing these lesions by direct irradiation. We show that all lesions are efficiently repaired except for the T(Dew)T lesion, which cannot be cleaved by the repair enzyme under our conditions. A crystal structure of a T(6-4)C lesion containing DNA duplex in complex with the (6-4) photolyase from Drosophila melanogaster provides insight into the molecular recognition event of a cytosine derived photolesion for the first time. In light of the previously postulated repair mechanism, which involves rearrangement of the (6-4) lesions into strained four-membered ring repair intermediates, it is surprising that the not rearranged T(6-4)C lesion is observed in the active site. The structure, therefore, provides additional support for the newly postulated repair mechanism that avoids this rearrangement step and argues for a direct electron injection into the lesion as the first step of the repair reaction performed by (6-4) DNA photolyases.
Crystal Structure of the T(6-4)C Lesion in Complex with a (6-4) DNA Photolyase and Repair of UV-Induced (6-4) and Dewar Photolesions.,Glas AF, Schneider S, Maul MJ, Hennecke U, Carell T Chemistry. 2009 Aug 31. PMID:19722240[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Glas AF, Schneider S, Maul MJ, Hennecke U, Carell T. Crystal Structure of the T(6-4)C Lesion in Complex with a (6-4) DNA Photolyase and Repair of UV-Induced (6-4) and Dewar Photolesions. Chemistry. 2009 Aug 31. PMID:19722240 doi:10.1002/chem.200901004
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