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| | ==Structure of the methyltransferase domain from Modoc Virus, a Flavivirus with No Known Vector (NKV)== | | ==Structure of the methyltransferase domain from Modoc Virus, a Flavivirus with No Known Vector (NKV)== |
| - | <StructureSection load='2wa2' size='340' side='right' caption='[[2wa2]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='2wa2' size='340' side='right'caption='[[2wa2]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2wa2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Modv Modv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WA2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WA2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2wa2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Modoc_virus Modoc virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WA2 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wa1|2wa1]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wa2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wa2 OCA], [http://pdbe.org/2wa2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wa2 RCSB], [http://www.ebi.ac.uk/pdbsum/2wa2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wa2 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wa2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wa2 OCA], [https://pdbe.org/2wa2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wa2 RCSB], [https://www.ebi.ac.uk/pdbsum/2wa2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wa2 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8QL64_9FLAV Q8QL64_9FLAV] Component of the viral RNA replication complex that functions in virion assembly and antagonizes the host immune response.[ARBA:ARBA00024317] Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.[ARBA:ARBA00003504] Serine protease subunit NS2B: Required cofactor for the serine protease function of NS3.[PROSITE-ProRule:PRU00859] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Modv]] | + | [[Category: Large Structures]] |
| - | [[Category: Jansson, A M]]
| + | |
| - | [[Category: Johansson, P]]
| + | |
| - | [[Category: Jones, T A]]
| + | |
| - | [[Category: 2'-o-methyltransferase]]
| + | |
| - | [[Category: Envelope protein]]
| + | |
| - | [[Category: Flavivirus]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Methyltransferase]]
| + | |
| | [[Category: Modoc virus]] | | [[Category: Modoc virus]] |
| - | [[Category: N7-methyltransferase]] | + | [[Category: Jansson AM]] |
| - | [[Category: S-adenosyl-l- methionine]] | + | [[Category: Johansson P]] |
| - | [[Category: Transferase]] | + | [[Category: Jones TA]] |
| - | [[Category: Transmembrane]]
| + | |
| - | [[Category: Virion]]
| + | |
| Structural highlights
Function
Q8QL64_9FLAV Component of the viral RNA replication complex that functions in virion assembly and antagonizes the host immune response.[ARBA:ARBA00024317] Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.[ARBA:ARBA00003504] Serine protease subunit NS2B: Required cofactor for the serine protease function of NS3.[PROSITE-ProRule:PRU00859]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Modoc virus (MODV) is a flavivirus with no known vector (NKV). Evolutionary studies have shown that the viruses in the MODV group have evolved in association with mammals (bats, rodents) without transmission by an arthropod vector. MODV methyltransferase is the first enzyme from this evolutionary branch to be structurally characterized. The high-resolution structure of the methyltransferase domain of the MODV NS5 protein (MTase(MODV)) was determined. The protein structure was solved in the apo form and in complex with its cofactor S-adenosyl-L-methionine (SAM). Although it belongs to a separate evolutionary branch, MTase(MODV) shares structural characteristics with flaviviral MTases from the other branches. Its capping machinery is a relatively new target in flaviviral drug development and the observed structural conservation between the three flaviviral branches indicates that it may be possible to identify a drug that targets a range of flaviviruses. The structural conservation also supports the choice of MODV as a possible model for flavivirus studies.
Structure of the methyltransferase domain from the Modoc virus, a flavivirus with no known vector.,Jansson AM, Jakobsson E, Johansson P, Lantez V, Coutard B, de Lamballerie X, Unge T, Jones TA Acta Crystallogr D Biol Crystallogr. 2009 Aug;65(Pt 8):796-803. Epub 2009, Jul 10. PMID:19622863[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jansson AM, Jakobsson E, Johansson P, Lantez V, Coutard B, de Lamballerie X, Unge T, Jones TA. Structure of the methyltransferase domain from the Modoc virus, a flavivirus with no known vector. Acta Crystallogr D Biol Crystallogr. 2009 Aug;65(Pt 8):796-803. Epub 2009, Jul 10. PMID:19622863 doi:10.1107/S0907444909017260
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