2wkj

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==Crystal structure of the E192N mutant of E. Coli N-acetylneuraminic acid lyase in complex with pyruvate at 1.45A resolution in space group P212121==
==Crystal structure of the E192N mutant of E. Coli N-acetylneuraminic acid lyase in complex with pyruvate at 1.45A resolution in space group P212121==
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<StructureSection load='2wkj' size='340' side='right' caption='[[2wkj]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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<StructureSection load='2wkj' size='340' side='right'caption='[[2wkj]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2wkj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WKJ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2wkj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WKJ FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KPI:(2S)-2-AMINO-6-[(1-HYDROXY-1-OXO-PROPAN-2-YLIDENE)AMINO]HEXANOIC+ACID'>KPI</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=KPI:(2S)-2-AMINO-6-[(1-HYDROXY-1-OXO-PROPAN-2-YLIDENE)AMINO]HEXANOIC+ACID'>KPI</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1nal|1nal]], [[1hl2|1hl2]], [[1fdz|1fdz]], [[1fdy|1fdy]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkj OCA], [https://pdbe.org/2wkj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wkj RCSB], [https://www.ebi.ac.uk/pdbsum/2wkj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wkj ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylneuraminate_lyase N-acetylneuraminate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.3 4.1.3.3] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkj OCA], [http://pdbe.org/2wkj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wkj RCSB], [http://www.ebi.ac.uk/pdbsum/2wkj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wkj ProSAT]</span></td></tr>
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</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NANA_ECOLI NANA_ECOLI] Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate.[HAMAP-Rule:MF_01237]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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==See Also==
==See Also==
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*[[Aldolase|Aldolase]]
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*[[N-acetylneuraminate lyase 3D structures|N-acetylneuraminate lyase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus coli migula 1895]]
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[[Category: Escherichia coli]]
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[[Category: N-acetylneuraminate lyase]]
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[[Category: Large Structures]]
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[[Category: Berry, A]]
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[[Category: Berry A]]
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[[Category: Campeotto, I]]
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[[Category: Campeotto I]]
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[[Category: Carr, S B]]
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[[Category: Carr SB]]
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[[Category: Nelson, A S]]
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[[Category: Nelson AS]]
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[[Category: Pearson, A R]]
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[[Category: Pearson AR]]
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[[Category: Phillips, S E.V]]
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[[Category: Phillips SEV]]
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[[Category: Trinh, C H]]
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[[Category: Trinh CH]]
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[[Category: Aldolase]]
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[[Category: Carbohydrate metabolism]]
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[[Category: Directed evolution]]
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[[Category: Lyase]]
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[[Category: N-acetylneuraminic acid lyase]]
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[[Category: Schiff base]]
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[[Category: Sialic acid mimetic]]
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Current revision

Crystal structure of the E192N mutant of E. Coli N-acetylneuraminic acid lyase in complex with pyruvate at 1.45A resolution in space group P212121

PDB ID 2wkj

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