Pseudouridine synthase

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<StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA and pseudouridine monophosphate [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'>
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<StructureSection load='1ze2' size='400' side='right' caption='tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate [[1ze2]]' scene='46/465439/Cv/1' pspeed='8'>
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== Function ==
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__TOC__
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'''tRNA pseudouridine synthase''' (PUS) catalyzes the conversion of tRNA uridine to tRNA pseudouridine<ref>PMID:14566049</ref>. PUS are active in the ribosomal large subunit (Rlu) and ribosomal small subunit (Rsu). '''Probable tRNA pseudouridine synthase''' (PPUS) is a similar enzyme.
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==Function==
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[[Pseudouridine]] is an abundant modified nucleoside found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the bond between base and ribose in uridine. There are two major types of enzymes that catalyze this reaction:
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== Structural highlights ==
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* [[RNA-guided pseudouridine synthase]] -- Some ribonucleoproteins (complexes of proteins and small RNAs) have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to select the site of modification through base-pairing with the target RNA molecule.
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The conversion of uridine to pseudouridine causes conformational change of PUS<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/3'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]).
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</StructureSection>
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== 3D Structures of tRNA pseudouridine synthase==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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* [[Guide-independent Pseudouridine synthase|Guide-independent pseudouridine synthase]] -- The alternative to using the RNA-guided mechanism for pseudouridine formation is a [[Guide-independent Pseudouridine synthase|guide-independent mechanism]] acting through protein-only enzymes to catalyze the isomerization.
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*tRNA pseudouridine synthase
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== Structural highlights ==
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The conversion of uridine to pseudouridine is accompanied by a conformational change of pseudouridine synthase<ref>PMID:14990747</ref>. <scene name='46/465439/Cv/4'>tRNA pseudouridine synthase binding with pseudouridine monophosphate</scene> ([[1ze2]]).
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== 3D Structures of pseudouridine synthase ==
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[[Pseudouridine synthase 3D structures]]
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**[[1dj0]] – EcPUS I – ''Escherichia coli''<BR />
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</StructureSection>
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**[[4iqm]], [[4its]], [[4j37]], [[4nz6]] - hPUS Pus1 catalytic domain - human<br />
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**[[4nz7]] - hPUS Pus1 catalytic domain (mutant)<br />
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**[[1vs3]] – PUS TruA – ''Thermus thermophilus''<BR />
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**[[1sgv]] – PUS TruB – ''Mycobacterium tuberculosis''<BR />
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**[[1si7]], [[1szw]], [[1sb7]] - EcPUS TruD
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*tRNA pseudouridine synthase complex with RNA
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**[[1k8w]] – EcTruB + stem-loop RNA<BR />
 
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**[[1zl3]] - EcTruB (mutant) + stem-loop RNA<BR />
 
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**[[2nqp]], [[2nr0]], [[2nre]] – EcTruA + Leu-tRNA<BR />
 
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**[[3hjw]], [[3lwo]], [[3lwp]], [[3lwq]], [[3lwr]], [[3lwv]] – PfPUS Cbf5 + ribosome biogenesis protein Nop10 + ribosomal protein L7 + RNA – ''Pyrococcus furiosus''<BR />
 
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**[[3mqk]] - PfPUS Cbf5 + ribosome biogenesis protein Nop10 + small nuclear RNP Gar1-like protein + RNA<BR />
 
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**[[3hjy]] - PfPUS Cbf5 + ribosome biogenesis protein NOP10 + RNA<BR />
 
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**[[1ze2]] - TmPUS B + RNA<BR />
 
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**[[2ab4]] - TmPUS B (mutant) + RNA<BR />
 
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**[[1r3e]], [[1r3f]] - EcTruB + RNA<BR />
 
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}}
 
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== References ==
 
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<references/>
 
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

tRNA pseudouridine synthase complex with RNA that contains pseudouridine monophosphate 1ze2

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