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6gp1
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of mEos4b in the red long-lived dark state== | |
| + | <StructureSection load='6gp1' size='340' side='right'caption='[[6gp1]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6gp1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lobophyllia_hemprichii Lobophyllia hemprichii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GP1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.504Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7R6:2-[(4E)-4-[(4-hydroxyphenyl)methylidene]-2-[(E)-2-(1H-imidazol-4-yl)ethenyl]-5-oxidanylidene-imidazol-1-yl]ethanoic+acid'>7R6</scene>, <scene name='pdbligand=NFA:PHENYLALANINE+AMIDE'>NFA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gp1 OCA], [https://pdbe.org/6gp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gp1 RCSB], [https://www.ebi.ac.uk/pdbsum/6gp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gp1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q5S6Z9_LOBHE Q5S6Z9_LOBHE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort. | ||
| - | + | Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM.,De Zitter E, Thedie D, Monkemoller V, Hugelier S, Beaudouin J, Adam V, Byrdin M, Van Meervelt L, Dedecker P, Bourgeois D Nat Methods. 2019 Jul 8. pii: 10.1038/s41592-019-0462-3. doi:, 10.1038/s41592-019-0462-3. PMID:31285624<ref>PMID:31285624</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6gp1" style="background-color:#fffaf0;"></div> |
| - | [[Category: Adam | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] |
| - | [[Category: De Zitter | + | == References == |
| - | [[Category: Dedecker | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Lobophyllia hemprichii]] | ||
| + | [[Category: Adam V]] | ||
| + | [[Category: Bourgeois D]] | ||
| + | [[Category: Byrdin M]] | ||
| + | [[Category: De Zitter E]] | ||
| + | [[Category: Dedecker P]] | ||
| + | [[Category: Van Meervelt L]] | ||
Current revision
Structure of mEos4b in the red long-lived dark state
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