6mlf
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6mlf is ON HOLD Authors: Beamer, L., Stiers, K. Description: Crystal structure of X. citri phosphoglucomutase in complex with 6-fluoro glucose 1-ph...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of X. citri phosphoglucomutase in complex with 6-fluoro glucose 1-phosphate== | |
| + | <StructureSection load='6mlf' size='340' side='right'caption='[[6mlf]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6mlf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_citri Xanthomonas citri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MLF FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JV4:[(2~{R},3~{R},4~{S},5~{S},6~{S})-6-(fluoranylmethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]+dihydrogen+phosphate'>JV4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mlf OCA], [https://pdbe.org/6mlf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mlf RCSB], [https://www.ebi.ac.uk/pdbsum/6mlf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mlf ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | alpha-Phosphomannomutase/phosphoglucomutase (alphaPMM/PGM) from P. aeruginosa is involved in bacterial cell wall assembly and is implicated in P. aeruginosa virulence, yet few studies have addressed alphaPMM/PGM inhibition from this important Gram-negative bacterial human pathogen. Four structurally different alpha-d-glucopyranose 1-phosphate (alphaG1P) derivatives including 1-C-fluoromethylated analogues (1-3), 1,2-cyclic phosph(on)ate analogues (4-6), isosteric methylene phosphono analogues (7 and 8), and 6-fluoro-alphaG1P (9), were synthesized and assessed as potential time-dependent or reversible alphaPMM/PGM inhibitors. The resulting kinetic data were consistent with the crystallographic structures of the highly homologous Xanthomonas citri alphaPGM with inhibitors 3 and 7-9 binding to the enzyme active site (1.65-1.9 A). These structural and kinetic insights will enhance the design of future alphaPMM/PGM inhibitors. | ||
| - | + | Inhibitory Evaluation of alphaPMM/PGM from Pseudomonas aeruginosa: Chemical Synthesis, Enzyme Kinetics, and Protein Crystallographic Study.,Zhu JS, Stiers KM, Soleimani E, Groves BR, Beamer LJ, Jakeman DL J Org Chem. 2019 Jul 16. doi: 10.1021/acs.joc.9b01305. PMID:31264865<ref>PMID:31264865</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6mlf" style="background-color:#fffaf0;"></div> |
| - | [[Category: Beamer | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Xanthomonas citri]] | ||
| + | [[Category: Beamer L]] | ||
| + | [[Category: Stiers K]] | ||
Current revision
Crystal structure of X. citri phosphoglucomutase in complex with 6-fluoro glucose 1-phosphate
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