This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

5zb6

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

NMR structure of UVI31+

Structural highlights

5zb6 is a 1 chain structure with sequence from Chlamydomonas reinhardtii. This structure supersedes the now removed PDB entry 2ma0. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Chlamydomonas reinhardtii is a single celled alga that undergoes apoptosis in response to UV-C irradiation. UVI31+, a novel UV-inducible DNA endonuclease in C. reinhardtii, which normally localizes near cell wall and pyrenoid regions, gets redistributed into punctate foci within the whole chloroplast, away from the pyrenoid, upon UV-stress. Solution NMR structure of the first putative UV inducible endonuclease UVI31+ revealed an alpha1-beta1-beta2-alpha2-alpha3-beta3 fold similar to BolA and type II KH-domain ubiquitous protein families. Three alpha-helices of UVI31+ constitute one side of the protein surface, which are packed to the other side, made of three-stranded beta-sheet, with intervening hydrophobic residues. A twenty-three residues long polypeptide stretch (D54-H76) connecting beta1 and beta2 strands is found to be highly flexible. Interestingly, UVI31+ recognizes the DNA primarily through its beta-sheet. We propose that the catalytic triad residues involving Ser114, His95 and Thr116 facilitate DNA endonuclease activity of UVI31+. Further, decreased endonuclease activity of the S114A mutant is consistent with the direct participation of Ser114 in the catalysis. This study provides the first structural description of a plant chloroplast endonuclease that is regulated by UV-stress response.

Structural characterization of a novel KH-domain containing plant chloroplast endonuclease.,Rout AK, Singh H, Patel S, Raghvan V, Gautam S, Minda R, Rao BJ, Chary KVR Sci Rep. 2018 Sep 13;8(1):13750. doi: 10.1038/s41598-018-31142-w. PMID:30214061^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rout AK, Singh H, Patel S, Raghvan V, Gautam S, Minda R, Rao BJ, Chary KVR. Structural characterization of a novel KH-domain containing plant chloroplast endonuclease. Sci Rep. 2018 Sep 13;8(1):13750. doi: 10.1038/s41598-018-31142-w. PMID:30214061 doi:http://dx.doi.org/10.1038/s41598-018-31142-w

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5zb6"

@@ Line 1: / Line 1: @@
 ==NMR structure of UVI31+==
-<StructureSection load='5zb6' size='340' side='right' caption='[[5zb6]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+<StructureSection load='5zb6' size='340' side='right'caption='[[5zb6]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5zb6]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ma0 2ma0]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZB6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZB6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5zb6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ma0 2ma0]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZB6 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zb6 OCA], [http://pdbe.org/5zb6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zb6 RCSB], [http://www.ebi.ac.uk/pdbsum/5zb6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zb6 ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zb6 OCA], [https://pdbe.org/5zb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zb6 RCSB], [https://www.ebi.ac.uk/pdbsum/5zb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zb6 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 19: / Line 19: @@
 __TOC__
 </StructureSection>
-[[Category: Chary, K V]]
+[[Category: Chlamydomonas reinhardtii]]
-[[Category: Patel, S]]
+[[Category: Large Structures]]
-[[Category: Rao, B J]]
+[[Category: Chary KV]]
-[[Category: Rout, A K]]
+[[Category: Patel S]]
-[[Category: Plant protein]]
+[[Category: Rao BJ]]
-[[Category: Structure from cyana 3 0]]
+[[Category: Rout AK]]

5zb6

From Proteopedia

Current revision

NMR structure of UVI31+

Structural highlights

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox