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6b80

From Proteopedia

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Current revision

Crystal structure of myotoxin II from Bothrops moojeni complexed to myristic acid

Structural highlights

6b80 is a 2 chain structure with sequence from Bothrops moojeni. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.949Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PA2H2_BOTMO Snake venom phospholipase A2 homolog that lack enzymatic activity. Displays myotoxin and edema-inducing activities.

Publication Abstract from PubMed

The myotoxic mechanism for PLA2-like toxins has been proposed recently to be initiated by an allosteric change induced by a fatty acid binding to the protein, leading to the alignment of the membrane docking site (MDoS) and membrane disrupting site (MDiS). Previous structural studies performed by us demonstrated that MjTX-II, a PLA2-like toxin isolated from Bothrops moojeni, presents a different mode of ligand-interaction caused by natural amino acid substitutions and an insertion. Herein, we present four crystal structures of MjTX-II, in its apo state and complexed with fatty acids of different lengths. Analyses of these structures revealed slightly different oligomeric conformations but with both MDoSs in an arrangement that resembles an active-state PLA2-like structure. To explore the structural transitions between apo protein and fatty-acid complexes, we performed Normal Mode Molecular Dynamics simulations, revealing that oligomeric conformations of MjTX-II/fatty acid complexes may be reached in solution by the apo structure. Similar simulations with typical PLA2-like structures demonstrated that this transition is not possible without the presence of fatty acids. Thus, we hypothesize that MjTX-II does not require fatty acids to be active, although these ligands may eventually help in its stabilization by the formation of hydrogen bonds. Therefore, these results complement previous findings for MjTX-II and help us understand its particular ligand-binding properties and, more importantly, its particular mechanism of action, with a possible impact on the design of structure-based inhibitors for PLA2-like toxins in general.

Structural evidence for a fatty acid-independent myotoxic mechanism for a phospholipase A2-like toxin.,Salvador GHM, Dos Santos JI, Borges RJ, Fontes MRM Biochim Biophys Acta Proteins Proteom. 2018 Mar;1866(3):473-481. doi:, 10.1016/j.bbapap.2017.12.008. Epub 2017 Dec 27. PMID:29287778^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Salvador GHM, Dos Santos JI, Borges RJ, Fontes MRM. Structural evidence for a fatty acid-independent myotoxic mechanism for a phospholipase A2-like toxin. Biochim Biophys Acta Proteins Proteom. 2018 Mar;1866(3):473-481. doi:, 10.1016/j.bbapap.2017.12.008. Epub 2017 Dec 27. PMID:29287778 doi:http://dx.doi.org/10.1016/j.bbapap.2017.12.008

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6b80"

Categories: Bothrops moojeni | Large Structures | Fontes MRM | Salvador GHM | Dos Santos JI

@@ Line 1: / Line 1: @@
 ==Crystal structure of myotoxin II from Bothrops moojeni complexed to myristic acid==
-<StructureSection load='6b80' size='340' side='right' caption='[[6b80]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
+<StructureSection load='6b80' size='340' side='right'caption='[[6b80]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6b80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bothrops_moojeni Bothrops moojeni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B80 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6b80]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bothrops_moojeni Bothrops moojeni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B80 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene>, <scene name='pdbligand=PE4:2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>PE4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.949&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kf3|4kf3]], [[4yv5|4yv5]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MYR:MYRISTIC+ACID'>MYR</scene>, <scene name='pdbligand=PE4:2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>PE4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b80 OCA], [http://pdbe.org/6b80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b80 RCSB], [http://www.ebi.ac.uk/pdbsum/6b80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b80 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b80 OCA], [https://pdbe.org/6b80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b80 RCSB], [https://www.ebi.ac.uk/pdbsum/6b80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b80 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PA2H2_BOTMO PA2H2_BOTMO]] Snake venom phospholipase A2 homolog that lack enzymatic activity. Displays myotoxin and edema-inducing activities.
+[https://www.uniprot.org/uniprot/PA2H2_BOTMO PA2H2_BOTMO] Snake venom phospholipase A2 homolog that lack enzymatic activity. Displays myotoxin and edema-inducing activities.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 6b80" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Phospholipase A2 homolog|Phospholipase A2 homolog]]
 == References ==
 <references/>
@@ Line 24: / Line 27: @@
 </StructureSection>
 [[Category: Bothrops moojeni]]
-[[Category: Fontes, M R.M]]
+[[Category: Large Structures]]
-[[Category: Salvador, G H.M]]
+[[Category: Fontes MRM]]
-[[Category: Santos, J I.dos]]
+[[Category: Salvador GHM]]
-[[Category: Myotoxin ii]]
+[[Category: Dos Santos JI]]
-[[Category: Phospholipase a2-like]]
-[[Category: Toxin]]

6b80

From Proteopedia

Current revision

Crystal structure of myotoxin II from Bothrops moojeni complexed to myristic acid

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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