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| | ==Bosea sp Root 381 apo GapR structure== | | ==Bosea sp Root 381 apo GapR structure== |
| - | <StructureSection load='6cfy' size='340' side='right' caption='[[6cfy]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='6cfy' size='340' side='right'caption='[[6cfy]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6cfy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bosea_sp._root381 Bosea sp. root381]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CFY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CFY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6cfy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bosea_sp._Root381 Bosea sp. Root381]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CFY FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ASE63_04290 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1736524 Bosea sp. Root381])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cfy OCA], [http://pdbe.org/6cfy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cfy RCSB], [http://www.ebi.ac.uk/pdbsum/6cfy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cfy ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cfy OCA], [https://pdbe.org/6cfy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cfy RCSB], [https://www.ebi.ac.uk/pdbsum/6cfy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cfy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0Q9HY32_9HYPH A0A0Q9HY32_9HYPH] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bosea sp. root381]] | + | [[Category: Bosea sp. Root381]] |
| - | [[Category: Schumacherr, M A]] | + | [[Category: Large Structures]] |
| - | [[Category: Caulobacter]] | + | [[Category: Schumacherr MA]] |
| - | [[Category: Dna binding]]
| + | |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Nap]]
| + | |
| - | [[Category: Topoisomerase]]
| + | |
| Structural highlights
Function
A0A0Q9HY32_9HYPH
Publication Abstract from PubMed
When DNA is unwound during replication, it becomes overtwisted and forms positive supercoils in front of the translocating DNA polymerase. Unless removed or dissipated, this superhelical tension can impede replication elongation. Topoisomerases, including gyrase and topoisomerase IV in bacteria, are required to relax positive supercoils ahead of DNA polymerase but may not be sufficient for replication. Here, we find that GapR, a chromosome structuring protein in Caulobacter crescentus, is required to complete DNA replication. GapR associates in vivo with positively supercoiled chromosomal DNA, and our biochemical and structural studies demonstrate that GapR forms a dimer-of-dimers that fully encircles overtwisted DNA. Further, we show that GapR stimulates gyrase and topo IV to relax positive supercoils, thereby enabling DNA replication. Analogous chromosome structuring proteins that locate to the overtwisted DNA in front of replication forks may be present in other organisms, similarly helping to recruit and stimulate topoisomerases during DNA replication.
A Bacterial Chromosome Structuring Protein Binds Overtwisted DNA to Stimulate Type II Topoisomerases and Enable DNA Replication.,Guo MS, Haakonsen DL, Zeng W, Schumacher MA, Laub MT Cell. 2018 Sep 7. pii: S0092-8674(18)31045-6. doi: 10.1016/j.cell.2018.08.029. PMID:30220456[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Guo MS, Haakonsen DL, Zeng W, Schumacher MA, Laub MT. A Bacterial Chromosome Structuring Protein Binds Overtwisted DNA to Stimulate Type II Topoisomerases and Enable DNA Replication. Cell. 2018 Sep 7. pii: S0092-8674(18)31045-6. doi: 10.1016/j.cell.2018.08.029. PMID:30220456 doi:http://dx.doi.org/10.1016/j.cell.2018.08.029
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