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6f45

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Crystal structure of the gp37-gp38 adhesin tip complex of the bacteriophage S16 long tail fiber

Structural highlights

6f45 is a 4 chain structure with sequence from Salmonella phage vB_SenM-S16. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.7035582Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

M1EBB2_BPS16 Vg38 is at the tip of the long tail fibers and serves as the phage recognition site for the cellular receptor.[ARBA:ARBA00003549]

Publication Abstract from PubMed

The ability of phages to infect specific bacteria has led to their exploitation as bio-tools for bacterial remediation and detection. Many phages recognize bacterial hosts via adhesin tips of their long tail fibers (LTFs). Adhesin sequence plasticity modulates receptor specificity, and thus primarily defines a phage's host range. Here we present the crystal structure of an adhesin (gp38) attached to a trimeric beta-helical tip (gp37) from the Salmonella phage S16 LTF. Gp38 contains rare polyglycine type II helices folded into a packed lattice, herein designated "PGII sandwich." Sequence variability within the domain is limited to surface-exposed helices and distal loops that form putative receptor-binding sites. In silico analyses revealed a prevalence of the adhesin architecture among T-even phages, excluding the archetypal T4 phage. Overall, S16 LTF provides a valuable model for understanding binding mechanisms of phage adhesins, and for engineering of phage adhesins with expandable or modulated host ranges.

Salmonella Phage S16 Tail Fiber Adhesin Features a Rare Polyglycine Rich Domain for Host Recognition.,Dunne M, Denyes JM, Arndt H, Loessner MJ, Leiman PG, Klumpp J Structure. 2018 Aug 16. pii: S0969-2126(18)30263-6. doi:, 10.1016/j.str.2018.07.017. PMID:30244968^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dunne M, Denyes JM, Arndt H, Loessner MJ, Leiman PG, Klumpp J. Salmonella Phage S16 Tail Fiber Adhesin Features a Rare Polyglycine Rich Domain for Host Recognition. Structure. 2018 Aug 16. pii: S0969-2126(18)30263-6. doi:, 10.1016/j.str.2018.07.017. PMID:30244968 doi:http://dx.doi.org/10.1016/j.str.2018.07.017

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6f45"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the gp37-gp38 adhesin tip complex of the bacteriophage S16 long tail fiber==
-<StructureSection load='6f45' size='340' side='right' caption='[[6f45]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+<StructureSection load='6f45' size='340' side='right'caption='[[6f45]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6f45]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_bacteriophage_s16 Salmonella bacteriophage s16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F45 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F45 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6f45]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_phage_vB_SenM-S16 Salmonella phage vB_SenM-S16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F45 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F45 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7035582&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f45 OCA], [http://pdbe.org/6f45 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f45 RCSB], [http://www.ebi.ac.uk/pdbsum/6f45 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f45 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f45 OCA], [https://pdbe.org/6f45 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f45 RCSB], [https://www.ebi.ac.uk/pdbsum/6f45 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f45 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/M1EBB2_BPS16 M1EBB2_BPS16] Vg38 is at the tip of the long tail fibers and serves as the phage recognition site for the cellular receptor.[ARBA:ARBA00003549]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Salmonella bacteriophage s16]]
+[[Category: Large Structures]]
-[[Category: Dunne, M]]
+[[Category: Salmonella phage vB_SenM-S16]]
-[[Category: Klumpp, J]]
+[[Category: Dunne M]]
-[[Category: Leiman, P]]
+[[Category: Klumpp J]]
-[[Category: Loessner, M J]]
+[[Category: Leiman P]]
-[[Category: Bacteriophage]]
+[[Category: Loessner MJ]]
-[[Category: Helical sandwich]]
-[[Category: Polyglycine]]
-[[Category: Tail fiber]]
-[[Category: Viral protein]]

6f45

From Proteopedia

Current revision

Crystal structure of the gp37-gp38 adhesin tip complex of the bacteriophage S16 long tail fiber

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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