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2xkn

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Crystal structure of the Fab fragment of the anti-EGFR antibody 7A7

Structural highlights

2xkn is a 4 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.4Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The EGF receptor is an important target of cancer immunotherapies. The 7A7 monoclonal antibody has been raised against the murine EGFR, but it cross-reacts with the human receptor. The results from experiments using immune-competent mice can therefore, in principle, be extrapolated to the corresponding scenario in humans. In this work we report the crystal structure of the 7A7 Fab at an effective resolution of 1.4A. The antibody binding site comprises a deep pocket, located at the interface between the light and heavy chains, with major contributions from CDR loops H1, H2, H3 and L1. Binding experiments show that 7A7 recognizes a site on the EGFR extracellular domain that is not accessible in its most stable conformations, but that becomes exposed upon treatment with a tyrosine kinase inhibitor. This suggests a recognition mechanism similar to that proposed for mAb 806.

Structure of the Fab fragment of the anti-murine EGFR antibody 7A7 and exploration of its receptor binding site.,Talavera A, Mackenzie J, Garrido G, Friemann R, Lopez-Requena A, Moreno E, Krengel U Mol Immunol. 2011 Jul;48(12-13):1578-85. Epub 2011 May 18. PMID:21592580^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Talavera A, Mackenzie J, Garrido G, Friemann R, Lopez-Requena A, Moreno E, Krengel U. Structure of the Fab fragment of the anti-murine EGFR antibody 7A7 and exploration of its receptor binding site. Mol Immunol. 2011 Jul;48(12-13):1578-85. Epub 2011 May 18. PMID:21592580 doi:10.1016/j.molimm.2011.03.016

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xkn"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Fab fragment of the anti-EGFR antibody 7A7==
-<StructureSection load='2xkn' size='340' side='right' caption='[[2xkn]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
+<StructureSection load='2xkn' size='340' side='right'caption='[[2xkn]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2xkn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XKN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2xkn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XKN FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PE4:2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>PE4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xkn OCA], [http://pdbe.org/2xkn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xkn RCSB], [http://www.ebi.ac.uk/pdbsum/2xkn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xkn ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PE4:2-{2-[2-(2-{2-[2-(2-ETHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHOXY]-ETHOXY}-ETHANOL'>PE4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xkn OCA], [https://pdbe.org/2xkn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xkn RCSB], [https://www.ebi.ac.uk/pdbsum/2xkn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xkn ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 23: / Line 24: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Friemann, R]]
+[[Category: Friemann R]]
-[[Category: Krengel, U]]
+[[Category: Krengel U]]
-[[Category: Mackenzie, J]]
+[[Category: Mackenzie J]]
-[[Category: Talavera, A]]
+[[Category: Talavera A]]
-[[Category: Immune system]]
-[[Category: Immunoglobulin]]

2xkn

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Current revision

Crystal structure of the Fab fragment of the anti-EGFR antibody 7A7

Structural highlights

Publication Abstract from PubMed

See Also

References

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