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| | ==Structural Determinants for Improved Thermal Stability of Designed Ankyrin Repeat Proteins With a Redesigned C-capping Module.== | | ==Structural Determinants for Improved Thermal Stability of Designed Ankyrin Repeat Proteins With a Redesigned C-capping Module.== |
| - | <StructureSection load='2xen' size='340' side='right' caption='[[2xen]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='2xen' size='340' side='right'caption='[[2xen]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2xen]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XEN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XEN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2xen]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XEN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XEN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MOH:METHANOL'>MOH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xeh|2xeh]], [[2xee|2xee]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MOH:METHANOL'>MOH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xen FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xen OCA], [http://pdbe.org/2xen PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xen RCSB], [http://www.ebi.ac.uk/pdbsum/2xen PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xen ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xen FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xen OCA], [https://pdbe.org/2xen PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xen RCSB], [https://www.ebi.ac.uk/pdbsum/2xen PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xen ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Synthetic construct sequences]] | + | [[Category: Large Structures]] |
| - | [[Category: Grutter, M]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Kramer, M]] | + | [[Category: Grutter M]] |
| - | [[Category: Mittl, P]] | + | [[Category: Kramer M]] |
| - | [[Category: Pluckthun, A]] | + | [[Category: Mittl P]] |
| - | [[Category: Wetzel, S K]] | + | [[Category: Pluckthun A]] |
| - | [[Category: Ankyrin]]
| + | [[Category: Wetzel SK]] |
| - | [[Category: De novo protein]]
| + | |
| - | [[Category: Design]]
| + | |
| - | [[Category: Protein engineering]]
| + | |
| - | [[Category: Protein-protein interaction]]
| + | |
| - | [[Category: Repeat protein]]
| + | |
| Structural highlights
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Designed ankyrin-repeat proteins (DARPins) that specifically bind to almost any target can be obtained by ribosome display or phage display from combinatorial libraries. Although DARPins are already very stable molecules, molecular dynamics simulations, equilibrium denaturation experiments, structural studies, and recent NMR experiments suggested that the unfolding of the original C-terminal capping repeat (C-cap), taken from a natural ankyrin repeat protein, limits the stability of the initial DARPin design. Several point mutations had been introduced to optimize the C-cap and were shown to indeed further increase the stability of DARPins. We now determined crystal structures of DARPins with one or three full-consensus internal repeats (NI(1)C or NI(3)C) between an N-cap and mutants of the C-cap. An NI(1)C mutant, in which the C-cap was only extended by three additional helix-forming residues, showed no structural change, but reduced B-factors in the C-cap. An NI(3)C C-cap mutant carrying five additional mutations in the interface to the preceding repeat, previously designed by using the consensus sequence as a guide, showed a rigid body movement of the C-cap towards the internal repeat. This movement results in an increased buried surface area, a superior surface complementarity and explains the improved stability in equilibrium unfolding, compared to the original C-cap. A C-cap mutant with three additional mutations introducing suitably spaced charged residues did not show formation of salt bridges, explaining why its stability was not increased further. These structural studies underline the importance of repeat coupling for stability and help in the further design of this protein family.
Structural Determinants for Improved Stability of Designed Ankyrin Repeat Proteins With a Redesigned C-capping Module.,Kramer MA, Wetzel SK, Pluckthun A, Mittl PR, Grutter MG J Mol Biol. 2010 Sep 16. PMID:20851127[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kramer MA, Wetzel SK, Pluckthun A, Mittl PR, Grutter MG. Structural Determinants for Improved Stability of Designed Ankyrin Repeat Proteins With a Redesigned C-capping Module. J Mol Biol. 2010 Sep 16. PMID:20851127 doi:10.1016/j.jmb.2010.09.023
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