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| | ==cadmium bound structure of SporoSAG== | | ==cadmium bound structure of SporoSAG== |
| - | <StructureSection load='2x28' size='340' side='right' caption='[[2x28]], [[Resolution|resolution]] 2.15Å' scene=''> | + | <StructureSection load='2x28' size='340' side='right'caption='[[2x28]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2x28]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgo Toxgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X28 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2x28]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X28 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wnk|2wnk]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x28 OCA], [http://pdbe.org/2x28 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x28 RCSB], [http://www.ebi.ac.uk/pdbsum/2x28 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x28 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x28 OCA], [https://pdbe.org/2x28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x28 RCSB], [https://www.ebi.ac.uk/pdbsum/2x28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x28 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
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| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x2/2x28_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x2/2x28_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Toxgo]] | + | [[Category: Large Structures]] |
| - | [[Category: Boulanger, M J]] | + | [[Category: Toxoplasma gondii]] |
| - | [[Category: Crawford, J]] | + | [[Category: Boulanger MJ]] |
| - | [[Category: Grigg, M E]] | + | [[Category: Crawford J]] |
| - | [[Category: Grujic, O]] | + | [[Category: Grigg ME]] |
| - | [[Category: Lamb, E]] | + | [[Category: Grujic O]] |
| - | [[Category: Wasmuth, J]] | + | [[Category: Lamb E]] |
| - | [[Category: Cell invasion]]
| + | [[Category: Wasmuth J]] |
| - | [[Category: Membrane protein]]
| + | |
| Structural highlights
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Toxoplasma gondii, the etiological agent of toxoplasmosis, utilizes stage-specific expression of antigenically distinct glycosylphosphatidylinositol-tethered surface coat proteins to promote and establish chronic infection. Of the three infective stages of T. gondii, sporozoites are encapsulated in highly infectious oocysts that have been linked to large scale outbreaks of toxoplasmosis. SporoSAG (surface antigen glycoprotein) is the dominant surface coat protein expressed on the surface of sporozoites. Using a bioinformatic approach, we show that SporoSAG clusters with the SAG2 subfamily of the SAG1-related superfamily (SRS) and is non-polymorphic among the 11 haplogroups of T. gondii strains. In contrast to the immunodominant SAG1 protein expressed on tachyzoites, SporoSAG is non-immunogenic during natural infection. We report the 1.60 A resolution crystal structure of SporoSAG solved using cadmium single anomalous dispersion. SporoSAG crystallized as a monomer and displays unique features of the SRS beta-sandwich fold relative to SAG1 and BSR4. Intriguingly, the structural diversity is localized to the upper sheets of the beta-sandwich fold and may have important implications for multimerization and host cell ligand recognition. The structure of SporoSAG also reveals an unexpectedly acidic surface that contrasts with the previously determined SAG1 and BSR4 structures where a basic surface is predicted to play a role in binding negatively charged glycosaminoglycans. Our structural and functional characterization of SporoSAG provides a rationale for the evolutionary divergence of this key SRS family member.
Structural and functional characterization of SporoSAG: a SAG2-related surface antigen from Toxoplasma gondii.,Crawford J, Lamb E, Wasmuth J, Grujic O, Grigg ME, Boulanger MJ J Biol Chem. 2010 Apr 16;285(16):12063-70. Epub 2010 Feb 17. PMID:20164173[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Crawford J, Lamb E, Wasmuth J, Grujic O, Grigg ME, Boulanger MJ. Structural and functional characterization of SporoSAG: a SAG2-related surface antigen from Toxoplasma gondii. J Biol Chem. 2010 Apr 16;285(16):12063-70. Epub 2010 Feb 17. PMID:20164173 doi:10.1074/jbc.M109.054866
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