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| | ==Crystal structure of the human Pygo2 PHD finger in complex with the B9L HD1 domain== | | ==Crystal structure of the human Pygo2 PHD finger in complex with the B9L HD1 domain== |
| - | <StructureSection load='2xb1' size='340' side='right' caption='[[2xb1]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='2xb1' size='340' side='right'caption='[[2xb1]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2xb1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XB1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2xb1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XB1 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xb1 OCA], [http://pdbe.org/2xb1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xb1 RCSB], [http://www.ebi.ac.uk/pdbsum/2xb1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xb1 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xb1 OCA], [https://pdbe.org/2xb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xb1 RCSB], [https://www.ebi.ac.uk/pdbsum/2xb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xb1 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PYGO2_HUMAN PYGO2_HUMAN]] Involved in signal transduction through the Wnt pathway. | + | [https://www.uniprot.org/uniprot/PYGO2_HUMAN PYGO2_HUMAN] Involved in signal transduction through the Wnt pathway.[https://www.uniprot.org/uniprot/BCL9L_HUMAN BCL9L_HUMAN] Transcriptional regulator that acts as an activator. Promotes beta-catenin transcriptional activity. Plays a role in tumorigenesis. Enhances the neoplastic transforming activity of CTNNB1 (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Bienz, M]] | + | [[Category: Large Structures]] |
| - | [[Category: Fiedler, M]] | + | [[Category: Bienz M]] |
| - | [[Category: Johnson, C M]] | + | [[Category: Fiedler M]] |
| - | [[Category: Miller, T C]] | + | [[Category: Johnson CM]] |
| - | [[Category: Rutherford, T J]] | + | [[Category: Miller TC]] |
| - | [[Category: Fusion protein]] | + | [[Category: Rutherford TJ]] |
| - | [[Category: Metal binding]]
| + | |
| - | [[Category: Signal transduction]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Wnt protein]]
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| Structural highlights
Function
PYGO2_HUMAN Involved in signal transduction through the Wnt pathway.BCL9L_HUMAN Transcriptional regulator that acts as an activator. Promotes beta-catenin transcriptional activity. Plays a role in tumorigenesis. Enhances the neoplastic transforming activity of CTNNB1 (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Zn-coordinated PHD fingers of Pygopus (Pygo) proteins are critical for beta-catenin-dependent transcriptional switches in normal and malignant tissues. They bind to methylated histone H3 tails, assisted by their BCL9 co-factors whose homology domain 1 (HD1) binds to the rear PHD surface. Although histone-binding residues are identical between the two human Pygo paralogs, we show here that Pygo2 complexes exhibit slightly higher binding affinities for methylated histone H3 tail peptides than Pygo1 complexes. We solved the crystal structure of the Pygo2 PHD-BCL9-2 HD1 complex, which revealed paralog-specific interactions in its PHD-HD1 interface that could contribute indirectly to its elevated affinity for the methylated histone H3 tail. Interestingly, using NMR spectroscopy, we discovered that HD1 binding to PHD triggers an allosteric communication with a conserved isoleucine residue that lines the binding channel for histone H3 threonine 3 (T3), the link between the two adjacent binding pockets accommodating histone H3 alanine 1 and methylated lysine 4, respectively. This modulates the surface of the T3 channel, providing a plausible explanation as to how BCL9 co-factors binding to Pygo PHD fingers impact indirectly on their histone binding affinity. Intriguingly, this allosteric modulation of the T3 channel is propagated through the PHD structural core by a highly conserved tryptophan, the signature residue defining the PHD subclass of Zn fingers, which suggests that other PHD proteins may also be assisted by co-factors in their decoding of modified histone H3 tails.
Allosteric remodelling of the histone H3 binding pocket in the Pygo2 PHD finger triggered by its binding to the B9L/BCL9 co-factor.,Miller TC, Rutherford TJ, Johnson CM, Fiedler M, Bienz M J Mol Biol. 2010 Sep 3;401(5):969-84. Epub 2010 Jul 14. PMID:20637214[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Miller TC, Rutherford TJ, Johnson CM, Fiedler M, Bienz M. Allosteric remodelling of the histone H3 binding pocket in the Pygo2 PHD finger triggered by its binding to the B9L/BCL9 co-factor. J Mol Biol. 2010 Sep 3;401(5):969-84. Epub 2010 Jul 14. PMID:20637214 doi:10.1016/j.jmb.2010.07.007
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