2qre

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[[Image:2qre.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (ZMP)==
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|PDB= 2qre |SIZE=350|CAPTION= <scene name='initialview01'>2qre</scene>, resolution 3.01&Aring;
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<StructureSection load='2qre' size='340' side='right'caption='[[2qre]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=AMZ:AMINOIMIDAZOLE+4-CARBOXAMIDE+RIBONUCLEOTIDE'>AMZ</scene>
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<table><tr><td colspan='2'>[[2qre]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QRE FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.01&#8491;</td></tr>
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|GENE= ssp2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMZ:AMINOIMIDAZOLE+4-CARBOXAMIDE+RIBONUCLEOTIDE'>AMZ</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qre OCA], [https://pdbe.org/2qre PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qre RCSB], [https://www.ebi.ac.uk/pdbsum/2qre PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qre ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2qr1|2QR1]], [[2qrc|2QRC]], [[2qrd|2QRD]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qre OCA], [http://www.ebi.ac.uk/pdbsum/2qre PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qre RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/SNF1_SCHPO SNF1_SCHPO]
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== Evolutionary Conservation ==
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'''Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (ZMP)'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qr/2qre_consurf.spt"</scriptWhenChecked>
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The AMP-activated protein kinase (AMPK), a sensor of cellular energy status found in all eukaryotes, responds to changes in intracellular adenosine nucleotide levels resulting from metabolic stresses. Here we describe crystal structures of a heterotrimeric regulatory core fragment from Schizosaccharomyces pombe AMPK in complex with ADP, ADP/AMP, ADP/ATP, and 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (AICAR phosphate, or ZMP), a well-characterized AMPK activator. Prior crystallographic studies had revealed a single site in the gamma subunit that binds either ATP or AMP within Bateman domain B. Here we show that ZMP binds at this site, mimicking the binding of AMP. An analogous site in Bateman domain A selectively accommodates ADP, which binds in a distinct manner that also involves direct ligation to elements from the beta subunit. These observations suggest a possible role for ADP in regulating AMPK response to changes in cellular energy status.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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2QRE is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRE OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qre ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structural insight into AMPK regulation: ADP comes into play., Jin X, Townley R, Shapiro L, Structure. 2007 Oct;15(10):1285-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17937917 17937917]
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</StructureSection>
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[[Category: Non-specific serine/threonine protein kinase]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
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[[Category: Jin, X.]]
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[[Category: Jin X]]
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[[Category: Shapiro, L.]]
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[[Category: Shapiro L]]
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[[Category: Townley, R.]]
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[[Category: Townley R]]
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[[Category: 5-aminoimidazole-4-carboxamide 1-beta-d-ribofuranotide]]
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[[Category: aicar phosphate]]
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[[Category: ampk]]
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[[Category: atp-binding]]
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[[Category: cbs domain]]
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[[Category: kinase]]
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[[Category: nucleotide-binding]]
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[[Category: serine/threonine-protein kinase]]
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[[Category: transferase]]
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[[Category: zmp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:52:29 2008''
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Current revision

Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (ZMP)

PDB ID 2qre

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