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6hs7
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6hs7 is ON HOLD Authors: Description: Category: Unreleased Structures) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Type VI membrane complex== | |
| + | <SX load='6hs7' size='340' side='right' viewer='molstar' caption='[[6hs7]], [[Resolution|resolution]] 4.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6hs7]] is a 25 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HS7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6HS7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6hs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hs7 OCA], [http://pdbe.org/6hs7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hs7 RCSB], [http://www.ebi.ac.uk/pdbsum/6hs7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hs7 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacteria have evolved macromolecular machineries that secrete effectors and toxins to survive and thrive in diverse environments. The type VI secretion system (T6SS) is a contractile machine that is related to Myoviridae phages. It is composed of a phage tail-like structure inserted in the bacterial cell envelope by a membrane complex (MC) comprising the TssJ, TssL and TssM proteins. We previously reported the low-resolution negative-stain electron microscopy structure of the enteroaggregative Escherichia coli MC and proposed a rotational 5-fold symmetry with a TssJ:TssL:TssM stoichiometry of 2:2:2. Here, cryo-electron tomography analyses of the T6SS MC confirm the 5-fold symmetry in situ and identify the regions of the structure that insert into the bacterial membranes. A high-resolution model obtained by single-particle cryo-electron microscopy highlights new features: five additional copies of TssJ, yielding a TssJ:TssL:TssM stoichiometry of 3:2:2, an 11-residue loop in TssM, protruding inside the lumen of the MC and constituting a functionally important periplasmic gate, and hinge regions. Based on these data, we propose an updated model on MC structure and dynamics during T6SS assembly and function. | ||
| - | + | In situ and high-resolution cryo-EM structure of a bacterial type VI secretion system membrane complex.,Rapisarda C, Cherrak Y, Kooger R, Schmidt V, Pellarin R, Logger L, Cascales E, Pilhofer M, Durand E, Fronzes R EMBO J. 2019 Mar 15. pii: embj.2018100886. doi: 10.15252/embj.2018100886. PMID:30877094<ref>PMID:30877094</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6hs7" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Fronzes, R]] | ||
| + | [[Category: Rapisarda, C]] | ||
| + | [[Category: Membrane complex]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Tether]] | ||
Current revision
Type VI membrane complex
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