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2qwl

From Proteopedia

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[[Image:2qwl.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of bovine hsc70 (1-394aa)in the ADP state==
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|PDB= 2qwl |SIZE=350|CAPTION= <scene name='initialview01'>2qwl</scene>, resolution 1.750&Aring;
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<StructureSection load='2qwl' size='340' side='right'caption='[[2qwl]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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<table><tr><td colspan='2'>[[2qwl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QWL FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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|GENE= HSPA8, HSC70 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qwl OCA], [https://pdbe.org/2qwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qwl RCSB], [https://www.ebi.ac.uk/pdbsum/2qwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qwl ProSAT]</span></td></tr>
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|RELATEDENTRY=[[2qwm|2QWM]], [[2qwn|2QWN]], [[2qwo|2QWO]], [[2qwp|2QWP]], [[2qwq|2QWQ]], [[2qwr|2QWR]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qwl OCA], [http://www.ebi.ac.uk/pdbsum/2qwl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qwl RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/HSP7C_BOVIN HSP7C_BOVIN] Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qw/2qwl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qwl ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of bovine hsc70 (1-394aa)in the ADP state'''
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==See Also==
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*[[Heat Shock Protein structures|Heat Shock Protein structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The many protein processing reactions of the ATP-hydrolyzing Hsp70s are regulated by J cochaperones, which contain J domains that stimulate Hsp70 ATPase activity and accessory domains that present protein substrates to Hsp70s. We report the structure of a J domain complexed with a J responsive portion of a mammalian Hsp70. The J domain activates ATPase activity by directing the linker that connects the Hsp70 nucleotide binding domain (NBD) and substrate binding domain (SBD) toward a hydrophobic patch on the NBD surface. Binding of the J domain to Hsp70 displaces the SBD from the NBD, which may allow the SBD flexibility to capture diverse substrates. Unlike prokaryotic Hsp70, the SBD and NBD of the mammalian chaperone interact in the ADP state. Thus, although both nucleotides and J cochaperones modulate Hsp70 NBD:linker and NBD:SBD interactions, the intrinsic persistence of those interactions differs in different Hsp70s and this may optimize their activities for different cellular roles.
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==About this Structure==
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2QWL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QWL OCA].
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==Reference==
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Structural basis of J cochaperone binding and regulation of Hsp70., Jiang J, Maes EG, Taylor AB, Wang L, Hinck AP, Lafer EM, Sousa R, Mol Cell. 2007 Nov 9;28(3):422-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17996706 17996706]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hinck, A P.]]
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[[Category: Hinck AP]]
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[[Category: Jiang, J.]]
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[[Category: Jiang J]]
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[[Category: Lafer, E M.]]
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[[Category: Lafer EM]]
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[[Category: Maes, E G.]]
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[[Category: Maes EG]]
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[[Category: Sousa, R.]]
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[[Category: Sousa R]]
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[[Category: Taylor, A B.]]
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[[Category: Taylor AB]]
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[[Category: Wang, L.]]
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[[Category: Wang L]]
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[[Category: atp-binding]]
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[[Category: chaperone]]
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[[Category: cytoplasm]]
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[[Category: nucleotide-binding]]
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[[Category: nucleus]]
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[[Category: phosphorylation]]
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[[Category: stress response]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:54:13 2008''
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Current revision

Crystal structure of bovine hsc70 (1-394aa)in the ADP state

PDB ID 2qwl

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