6ei0
From Proteopedia
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==Cytosolic copper storage protein Csp from Streptomyces lividans: apo form== | ==Cytosolic copper storage protein Csp from Streptomyces lividans: apo form== | ||
| - | <StructureSection load='6ei0' size='340' side='right' caption='[[6ei0]], [[Resolution|resolution]] 1.34Å' scene=''> | + | <StructureSection load='6ei0' size='340' side='right'caption='[[6ei0]], [[Resolution|resolution]] 1.34Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6ei0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EI0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EI0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ei0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_lividans_1326 Streptomyces lividans 1326]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EI0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EI0 FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAMN05428941_3460 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1200984 Streptomyces lividans 1326])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ei0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ei0 OCA], [http://pdbe.org/6ei0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ei0 RCSB], [http://www.ebi.ac.uk/pdbsum/6ei0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ei0 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ei0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ei0 OCA], [http://pdbe.org/6ei0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ei0 RCSB], [http://www.ebi.ac.uk/pdbsum/6ei0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ei0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Streptomyces lividans has a distinct dependence on the bioavailability of copper for its morphological development. A cytosolic copper resistance system is operative in S. lividans that serves to preclude deleterious copper levels. This system comprises of several CopZ-like copper chaperones and P1-type ATPases, predominantly under the transcriptional control of a metalloregulator from the copper sensitive operon repressor (CsoR) family. In the present study, we discover a new layer of cytosolic copper resistance in S. lividans that involves a protein belonging to the newly discovered family of copper storage proteins, which we have named Ccsp (cytosolic copper storage protein). From an evolutionary perspective, we find Ccsp homologues to be widespread in Bacteria and extend through into Archaea and Eukaryota. Under copper stress Ccsp is upregulated and consists of a homotetramer assembly capable of binding up to 80 cuprous ions (20 per protomer). X-ray crystallography reveals 18 cysteines, 3 histidines and 1 aspartate are involved in cuprous ion coordination. Loading of cuprous ions to Ccsp is a cooperative process with a Hill coefficient of 1.9 and a CopZ-like copper chaperone can transfer copper to Ccsp. A Deltaccsp mutant strain indicates that Ccsp is not required under initial copper stress in S. lividans, but as the CsoR/CopZ/ATPase efflux system becomes saturated, Ccsp facilitates a second level of copper tolerance. | ||
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| + | A cytosolic copper storage protein provides a second level of copper tolerance in Streptomyces lividans.,Straw ML, Chaplin AK, Hough MA, Paps J, Bavro VN, Wilson MT, Vijgenboom E, Worrall JAR Metallomics. 2018 Jan 24;10(1):180-193. doi: 10.1039/c7mt00299h. PMID:29292456<ref>PMID:29292456</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6ei0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptomyces lividans 1326]] | ||
[[Category: Chaplin, A K]] | [[Category: Chaplin, A K]] | ||
[[Category: Hough, M A]] | [[Category: Hough, M A]] | ||
Current revision
Cytosolic copper storage protein Csp from Streptomyces lividans: apo form
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