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| | ==Structure of Prokaryotic Transcription Factors== | | ==Structure of Prokaryotic Transcription Factors== |
| - | <StructureSection load='5nbc' size='340' side='right' caption='[[5nbc]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='5nbc' size='340' side='right'caption='[[5nbc]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5nbc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_tularense"_mccoy_and_chapin_1912 "bacterium tularense" mccoy and chapin 1912]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NBC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NBC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5nbc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Francisella_tularensis Francisella tularensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NBC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NBC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.699Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fur, AV531_00680, DR86_1441 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=263 "Bacterium tularense" McCoy and Chapin 1912])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nbc OCA], [http://pdbe.org/5nbc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nbc RCSB], [http://www.ebi.ac.uk/pdbsum/5nbc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nbc ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nbc OCA], [https://pdbe.org/5nbc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nbc RCSB], [https://www.ebi.ac.uk/pdbsum/5nbc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nbc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q5NIN6_FRATT Q5NIN6_FRATT] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacterium tularense mccoy and chapin 1912]] | + | [[Category: Francisella tularensis]] |
| - | [[Category: CARPENTIER, P]] | + | [[Category: Large Structures]] |
| - | [[Category: CAVAZZA, C]] | + | [[Category: Carpentier P]] |
| - | [[Category: MICHAUD-SORET, I]] | + | [[Category: Cavazza C]] |
| - | [[Category: PERARD, J]] | + | [[Category: Michaud-Soret I]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Perard J]] |
| - | [[Category: Virulence factor]]
| + | |
| Structural highlights
Function
Q5NIN6_FRATT
Publication Abstract from PubMed
Francisella tularensis is a Gram-negative bacterium causing tularaemia. Classified as possible bioterrorism agent, it may be transmitted to humans via animal infection or inhalation leading to severe pneumonia. Its virulence is related to iron homeostasis involving siderophore biosynthesis directly controlled at the transcription level by the ferric uptake regulator Fur, as presented here together with the first crystal structure of the tetrameric F. tularensis Fur in the presence of its physiological cofactor, Fe(2+). Through structural, biophysical, biochemical and modelling studies, we show that promoter sequences of F. tularensis containing Fur boxes enable this tetrameric protein to bind them by splitting it into two dimers. Furthermore, the critical role of F. tularensis Fur in virulence and pathogenesis is demonstrated with a fur-deleted mutant showing an attenuated virulence in macrophage-like cells and mice. Together, our study suggests that Fur is an attractive target of new antibiotics that attenuate the virulence of F. tularensis.
Structural and functional studies of the metalloregulator Fur identify a promoter-binding mechanism and its role in Francisella tularensis virulence.,Perard J, Nader S, Levert M, Arnaud L, Carpentier P, Siebert C, Blanquet F, Cavazza C, Renesto P, Schneider D, Maurin M, Coves J, Crouzy S, Michaud-Soret I Commun Biol. 2018 Jul 17;1:93. doi: 10.1038/s42003-018-0095-6. eCollection 2018. PMID:30271974[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Perard J, Nader S, Levert M, Arnaud L, Carpentier P, Siebert C, Blanquet F, Cavazza C, Renesto P, Schneider D, Maurin M, Coves J, Crouzy S, Michaud-Soret I. Structural and functional studies of the metalloregulator Fur identify a promoter-binding mechanism and its role in Francisella tularensis virulence. Commun Biol. 2018 Jul 17;1:93. doi: 10.1038/s42003-018-0095-6. eCollection 2018. PMID:30271974 doi:http://dx.doi.org/10.1038/s42003-018-0095-6
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