2r9k

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[[Image:2r9k.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of Misteltoe Lectin I in Complex with Phloretamide==
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|PDB= 2r9k |SIZE=350|CAPTION= <scene name='initialview01'>2r9k</scene>, resolution 2.70&Aring;
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<StructureSection load='2r9k' size='340' side='right'caption='[[2r9k]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Nag+Binding+Site+For+Residue+A+500'>AC1</scene>, <scene name='pdbsite=AC2:Nag+Binding+Site+For+Residue+B+602'>AC2</scene>, <scene name='pdbsite=AC3:Nag+Binding+Site+For+Residue+B+603'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+A+255'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+A+256'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+A+257'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Residue+B+3'>AC7</scene>, <scene name='pdbsite=AC8:Sgi+Binding+Site+For+Residue+A+600'>AC8</scene>, <scene name='pdbsite=AC9:Gol+Binding+Site+For+Residue+A+649'>AC9</scene>, <scene name='pdbsite=BC1:Gol+Binding+Site+For+Residue+A+650'>BC1</scene>, <scene name='pdbsite=BC2:Gol+Binding+Site+For+Residue+B+647'>BC2</scene> and <scene name='pdbsite=BC3:Gol+Binding+Site+For+Residue+B+648'>BC3</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SGI:3-(4-HYDROXYPHENYL)PROPANAMIDE'>SGI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[2r9k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R9K FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SGI:3-(4-HYDROXYPHENYL)PROPANAMIDE'>SGI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r9k OCA], [https://pdbe.org/2r9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r9k RCSB], [https://www.ebi.ac.uk/pdbsum/2r9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r9k ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1m2t|1M2T]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r9k OCA], [http://www.ebi.ac.uk/pdbsum/2r9k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2r9k RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/ML1_VISAL ML1_VISAL] The A chain is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits by removing adenine from position 4,324 of 28S rRNA. The B chain binds to cell receptors and probably facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (lectin activity). Inhibits growth of the human tumor cell line Molt4.<ref>PMID:15182350</ref> <ref>PMID:15001393</ref> <ref>PMID:1450445</ref>
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== Evolutionary Conservation ==
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'''Crystal Structure of Misteltoe Lectin I in Complex with Phloretamide'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r9/2r9k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r9k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The X-ray structure at 2.7A resolution of the complex between the European mistletoe lectin I (Viscum album, ML-I) and the plant growth hormone, 3-(p-hydroxyphenyl)-propionic acid amide (phloretamide, PA) from xylem sap has revealed the binding of PA at the so far undescribed hydrophobic cavity located between the two subunits of this ribosome-inhibiting protein. No such cavity is observed in related lectins. The binding of PA is achieved through interactions with the non-conserved residues Val228A, Leu230A, Arg388B, and the C-terminal Pro510B. It is conceivable that binding of PA to ML-I is part of a defence mechanism of the parasite against the host, whereby the parasite prevents the growth hormone of the host from interfering with its own regulatory system. The specific binding of PA to ML-I indicates that heterodimeric RIPs are multifunctional proteins whose functions in the cell have not yet been fully recognized and analyzed.
The X-ray structure at 2.7A resolution of the complex between the European mistletoe lectin I (Viscum album, ML-I) and the plant growth hormone, 3-(p-hydroxyphenyl)-propionic acid amide (phloretamide, PA) from xylem sap has revealed the binding of PA at the so far undescribed hydrophobic cavity located between the two subunits of this ribosome-inhibiting protein. No such cavity is observed in related lectins. The binding of PA is achieved through interactions with the non-conserved residues Val228A, Leu230A, Arg388B, and the C-terminal Pro510B. It is conceivable that binding of PA to ML-I is part of a defence mechanism of the parasite against the host, whereby the parasite prevents the growth hormone of the host from interfering with its own regulatory system. The specific binding of PA to ML-I indicates that heterodimeric RIPs are multifunctional proteins whose functions in the cell have not yet been fully recognized and analyzed.
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==About this Structure==
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The mistletoe lectin I--phloretamide structure reveals a new function of plant lectins.,Meyer A, Rypniewski W, Celewicz L, Erdmann VA, Voelter W, Singh TP, Genov N, Barciszewski J, Betzel Ch Biochem Biophys Res Commun. 2007 Dec 14;364(2):195-200. Epub 2007 Oct 5. PMID:17937929<ref>PMID:17937929</ref>
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2R9K is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R9K OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The mistletoe lectin I--phloretamide structure reveals a new function of plant lectins., Meyer A, Rypniewski W, Celewicz L, Erdmann VA, Voelter W, Singh TP, Genov N, Barciszewski J, Betzel Ch, Biochem Biophys Res Commun. 2007 Dec 14;364(2):195-200. Epub 2007 Oct 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17937929 17937929]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 2r9k" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Viscum album]]
[[Category: Viscum album]]
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[[Category: Betzel, C.]]
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[[Category: Betzel C]]
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[[Category: Celewicz, L.]]
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[[Category: Celewicz L]]
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[[Category: Erdmann, V A.]]
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[[Category: Erdmann VA]]
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[[Category: Meyer, A.]]
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[[Category: Meyer A]]
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[[Category: Rypniewski, W.]]
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[[Category: Rypniewski W]]
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[[Category: Voelter, W.]]
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[[Category: Voelter W]]
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[[Category: crystal structure]]
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[[Category: glycoprotein]]
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[[Category: hydrolase]]
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[[Category: lectin]]
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[[Category: ml-i]]
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[[Category: pharmaceutical]]
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[[Category: phloretamide]]
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[[Category: plant defense]]
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[[Category: polymorphism]]
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[[Category: protein synthesis inhibitor]]
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[[Category: toxin]]
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[[Category: viscum album]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:57:51 2008''
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Current revision

Crystal Structure of Misteltoe Lectin I in Complex with Phloretamide

PDB ID 2r9k

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