2rbi

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[[Image:2rbi.jpg|left|200px]]
 
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{{Structure
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==STRUCTURE OF BINASE MUTANT HIS 101 ASN==
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|PDB= 2rbi |SIZE=350|CAPTION= <scene name='initialview01'>2rbi</scene>, resolution 2.20&Aring;
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<StructureSection load='2rbi' size='340' side='right'caption='[[2rbi]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE= <scene name='pdbsite=1:Catalytic+Site+Residues'>1</scene>, <scene name='pdbsite=2:Mutated+Catalytic+Site+Residue'>2</scene> and <scene name='pdbsite=3:Main+Nucleotide+Binding+Residue'>3</scene>
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[2rbi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_intermedius Bacillus intermedius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RBI FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE= PHOA-BINASE HIS101ASN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1400 Bacillus intermedius])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rbi OCA], [https://pdbe.org/2rbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rbi RCSB], [https://www.ebi.ac.uk/pdbsum/2rbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rbi ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rbi OCA], [http://www.ebi.ac.uk/pdbsum/2rbi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2rbi RCSB]</span>
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[https://www.uniprot.org/uniprot/RN_BACIN RN_BACIN] This is a purine-specific ribonuclease.
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rb/2rbi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rbi ConSurf].
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<div style="clear:both"></div>
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'''STRUCTURE OF BINASE MUTANT HIS 101 ASN'''
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==See Also==
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Members of the microbial guanyl-specific ribonuclease family catalyse the endonucleolytic cleavage of single-stranded RNA in a two-step reaction involving transesterification to form a 2',3'-cyclic phosphate and its subsequent hydrolysis to yield the respective 3'-phosphate. The extracellular ribonuclease from Bacillus intermedius (binase, RNase Bi) shares a common mechanism for RNA hydrolysis with mammalian RNases. Two catalytic residues in the active site of binase, Glu72 and His101, are thought to be involved in general acid-general base catalysis of RNA cleavage. Using site-directed mutagenesis, binase mutants were produced containing amino acid substitutions H101N and H101T and their catalytic properties towards RNA, poly(I), poly(A), GpC and guanosine 2',3'-cyclic phosphate (cGMP) substrates were studied. The engineered mutant proteins are active in the transesterification step which produces the 2',3'-cyclic phosphate species but they have lost the ability to catalyse hydrolysis of the cyclic phosphate to give the 3' monophosphate product.
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==About this Structure==
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2RBI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_intermedius Bacillus intermedius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RBI OCA].
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==Reference==
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RNA cleavage without hydrolysis. Splitting the catalytic activities of binase with Asn101 and Thr101 mutations., Okorokov AL, Panov KI, Offen WA, Mukhortov VG, Antson AA, Karpeisky MYa, Wilkinson AJ, Dodson GG, Protein Eng. 1997 Mar;10(3):273-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9153077 9153077]
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[[Category: Bacillus intermedius]]
[[Category: Bacillus intermedius]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Offen, W A.]]
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[[Category: Offen WA]]
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[[Category: Okorokov, A L.]]
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[[Category: Okorokov AL]]
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[[Category: endoribonuclease]]
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[[Category: hydrolase]]
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[[Category: microbial extracellular ribonuclease]]
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[[Category: phosphodiester transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:58:34 2008''
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Current revision

STRUCTURE OF BINASE MUTANT HIS 101 ASN

PDB ID 2rbi

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