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| | ==Vps1 GTPase-BSE fusion complexed with GDP== | | ==Vps1 GTPase-BSE fusion complexed with GDP== |
| - | <StructureSection load='6di7' size='340' side='right' caption='[[6di7]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='6di7' size='340' side='right'caption='[[6di7]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6di7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DI7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DI7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6di7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DI7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0061810 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6di7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6di7 OCA], [http://pdbe.org/6di7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6di7 RCSB], [http://www.ebi.ac.uk/pdbsum/6di7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6di7 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6di7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6di7 OCA], [https://pdbe.org/6di7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6di7 RCSB], [https://www.ebi.ac.uk/pdbsum/6di7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6di7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/G0SFF0_CHATD G0SFF0_CHATD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chatd]] | + | [[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] |
| - | [[Category: Brady, T M]]
| + | [[Category: Large Structures]] |
| - | [[Category: Ford, M G.J]]
| + | [[Category: Brady TM]] |
| - | [[Category: Varlakhanova, N V]] | + | [[Category: Ford MGJ]] |
| - | [[Category: Drp]] | + | [[Category: Varlakhanova NV]] |
| - | [[Category: Dynamin]] | + | |
| - | [[Category: Dynamin-related protein]] | + | |
| - | [[Category: Endosome]]
| + | |
| - | [[Category: Gdp]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Vacuolar protein sorting 1]]
| + | |
| - | [[Category: Vacuole]]
| + | |
| - | [[Category: Vps1]]
| + | |
| Structural highlights
Function
G0SFF0_CHATD
Publication Abstract from PubMed
Dynamin-related proteins (DRPs) are large multidomain GTPases required for diverse membrane-remodeling events. DRPs self-assemble into helical structures, but how these structures are tailored to their cellular targets remains unclear. We demonstrate that the fungal DRP Vps1 primarily localizes to and functions at the endosomal compartment. We present crystal structures of a Vps1 GTPase-bundle signaling element (BSE) fusion in different nucleotide states to capture GTP hydrolysis intermediates and concomitant conformational changes. Using cryoEM, we determined the structure of full-length GMPPCP-bound Vps1. The Vps1 helix is more open and flexible than that of dynamin. This is due to further opening of the BSEs away from the GTPase domains. A novel interface between adjacent GTPase domains forms in Vps1 instead of the contacts between the BSE and adjacent stalks and GTPase domains as seen in dynamin. Disruption of this interface abolishes Vps1 function in vivo. Hence, Vps1 exhibits a unique helical architecture, highlighting structural flexibilities of DRP self-assembly.
Structures of the fungal dynamin-related protein Vps1 reveal a unique, open helical architecture.,Varlakhanova NV, Alvarez FJD, Brady TM, Tornabene BA, Hosford CJ, Chappie JS, Zhang P, Ford MGJ J Cell Biol. 2018 Aug 7. pii: jcb.201712021. doi: 10.1083/jcb.201712021. PMID:30087125[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Varlakhanova NV, Alvarez FJD, Brady TM, Tornabene BA, Hosford CJ, Chappie JS, Zhang P, Ford MGJ. Structures of the fungal dynamin-related protein Vps1 reveal a unique, open helical architecture. J Cell Biol. 2018 Aug 7. pii: jcb.201712021. doi: 10.1083/jcb.201712021. PMID:30087125 doi:http://dx.doi.org/10.1083/jcb.201712021
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