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| | ==Crystallographic structure of calcium loaded Calbindin-D28K.== | | ==Crystallographic structure of calcium loaded Calbindin-D28K.== |
| - | <StructureSection load='6fie' size='340' side='right' caption='[[6fie]], [[Resolution|resolution]] 1.51Å' scene=''> | + | <StructureSection load='6fie' size='340' side='right'caption='[[6fie]], [[Resolution|resolution]] 1.51Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6fie]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FIE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FIE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6fie]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FIE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FIE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.51Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2g9b|2g9b]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CALB1, CAB27 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fie FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fie OCA], [https://pdbe.org/6fie PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fie RCSB], [https://www.ebi.ac.uk/pdbsum/6fie PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fie ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fie FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fie OCA], [http://pdbe.org/6fie PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fie RCSB], [http://www.ebi.ac.uk/pdbsum/6fie PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fie ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CALB1_HUMAN CALB1_HUMAN]] Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. | + | [https://www.uniprot.org/uniprot/CALB1_HUMAN CALB1_HUMAN] Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Calbindin-D28K is a widely expressed calcium-buffering cytoplasmic protein that is involved in many physiological processes. It has been shown to interact with other proteins, suggesting a role as a calcium sensor. Many of the targets of calbindin-D28K are of therapeutic interest: for example, inositol monophosphatase, the putative target of lithium therapy in bipolar disorder. Presented here is the first crystal structure of human calbindin-D28K. There are significant deviations in the tertiary structure when compared with the NMR structure of rat calbindin-D28K (PDB entry 2g9b), despite 98% sequence identity. Small-angle X-ray scattering (SAXS) indicates that the crystal structure better predicts the properties of calbindin-D28K in solution compared with the NMR structure. Here, the first direct visualization of the calcium-binding properties of calbindin-D28K is presented. Four of the six EF-hands that make up the secondary structure of the protein contain a calcium-binding site. Two distinct conformations of the N-terminal EF-hand calcium-binding site were identified using long-wavelength calcium single-wavelength anomalous dispersion (SAD). This flexible region has previously been recognized as a protein-protein interaction interface. SAXS data collected in both the presence and absence of calcium indicate that there are no large structural differences in the globular structure of calbindin-D28K between the calcium-loaded and unloaded proteins.
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| - | The X-ray structure of human calbindin-D28K: an improved model.,Noble JW, Almalki R, Roe SM, Wagner A, Duman R, Atack JR Acta Crystallogr D Struct Biol. 2018 Oct 1;74(Pt 10):1008-1014. doi:, 10.1107/S2059798318011610. Epub 2018 Oct 2. PMID:30289411<ref>PMID:30289411</ref>
| + | ==See Also== |
| - | | + | *[[S100 proteins 3D structures|S100 proteins 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6fie" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Almalki, R]] | + | [[Category: Large Structures]] |
| - | [[Category: Atack, J R]] | + | [[Category: Almalki R]] |
| - | [[Category: Dumanc, R]] | + | [[Category: Atack JR]] |
| - | [[Category: Noble, J W]] | + | [[Category: Dumanc R]] |
| - | [[Category: Roe, S M]] | + | [[Category: Noble JW]] |
| - | [[Category: Wagner, A]] | + | [[Category: Roe SM]] |
| - | [[Category: Buffer]]
| + | [[Category: Wagner A]] |
| - | [[Category: Calcium binding protein]]
| + | |
| - | [[Category: Ef-hand]]
| + | |
| - | [[Category: Impase]]
| + | |
| - | [[Category: Metal binding protein]]
| + | |
