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| ==Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1== | | ==Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1== |
- | <StructureSection load='2z6o' size='340' side='right' caption='[[2z6o]], [[Resolution|resolution]] 1.60Å' scene=''> | + | <StructureSection load='2z6o' size='340' side='right'caption='[[2z6o]], [[Resolution|resolution]] 1.60Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[2z6o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z6O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Z6O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2z6o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z6O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z6O FirstGlance]. <br> |
- | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
- | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2z6p|2z6p]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
- | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UFC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z6o OCA], [https://pdbe.org/2z6o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z6o RCSB], [https://www.ebi.ac.uk/pdbsum/2z6o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z6o ProSAT]</span></td></tr> |
- | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
| + | |
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z6o OCA], [http://pdbe.org/2z6o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2z6o RCSB], [http://www.ebi.ac.uk/pdbsum/2z6o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2z6o ProSAT]</span></td></tr> | + | |
| </table> | | </table> |
| == Function == | | == Function == |
- | [[http://www.uniprot.org/uniprot/UFC1_HUMAN UFC1_HUMAN]] E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.<ref>PMID:15071506</ref> | + | [https://www.uniprot.org/uniprot/UFC1_HUMAN UFC1_HUMAN] E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.<ref>PMID:15071506</ref> |
| == Evolutionary Conservation == | | == Evolutionary Conservation == |
| [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| ==See Also== | | ==See Also== |
- | *[[Ferritin|Ferritin]] | + | *[[Ferritin 3D structures|Ferritin 3D structures]] |
| + | *[[Ubiquitin-fold modifier|Ubiquitin-fold modifier]] |
| == References == | | == References == |
| <references/> | | <references/> |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
- | [[Category: Human]] | + | [[Category: Homo sapiens]] |
- | [[Category: Ubiquitin--protein ligase]] | + | [[Category: Large Structures]] |
- | [[Category: Kawakami, T]] | + | [[Category: Kawakami T]] |
- | [[Category: Komatsu, M]] | + | [[Category: Komatsu M]] |
- | [[Category: Kominami, E]] | + | [[Category: Kominami E]] |
- | [[Category: Mizushima , T]] | + | [[Category: Mizushima T]] |
- | [[Category: Ogasahara, K]] | + | [[Category: Ogasahara K]] |
- | [[Category: Ozaki, Y]] | + | [[Category: Ozaki Y]] |
- | [[Category: Suzuki, A]] | + | [[Category: Suzuki A]] |
- | [[Category: Tanaka, K]] | + | [[Category: Tanaka K]] |
- | [[Category: Tatsumi, K]] | + | [[Category: Tatsumi K]] |
- | [[Category: Yamane, T]] | + | [[Category: Yamane T]] |
- | [[Category: Ligase]]
| + | |
- | [[Category: Polymorphism]]
| + | |
- | [[Category: Ubiquitin]]
| + | |
- | [[Category: Ubl]]
| + | |
- | [[Category: Ubl conjugation pathway]]
| + | |
- | [[Category: Ufc1]]
| + | |
- | [[Category: Ufm1]]
| + | |
| Structural highlights
Function
UFC1_HUMAN E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Ubiquitin and ubiquitin-like protein-conjugating enzymes play central roles in posttranslational modification processes. The ubiquitin-fold modifier 1 (Ufm1), one of a variety of ubiquitin-like modifiers, is covalently attached to target proteins via Uba5 and Ufm1-conjugating enzyme 1 (Ufc1), which are analogous to the E1 and E2 ubiquitylation enzymes. As Ufm1-related proteins are conserved in metazoa and plants, the Ufm1 system likely plays important roles in various multicellular organisms. Herein, we report the X-ray structure of human Ufc1 determined at 1.6 A resolution. The Ufc1 structure comprises a canonical E2 domain and an additional N-terminal domain. The Uba5 binding site on Ufc1 was assigned by structural comparison of Ufc1 and Ubc12 and related mutational analyses. In addition, we show that the N-terminal unique domain of Ufc1 contributes to thermal stability.
Crystal structure of Ufc1, the Ufm1-conjugating enzyme.,Mizushima T, Tatsumi K, Ozaki Y, Kawakami T, Suzuki A, Ogasahara K, Komatsu M, Kominami E, Tanaka K, Yamane T Biochem Biophys Res Commun. 2007 Nov 3;362(4):1079-84. Epub 2007 Aug 30. PMID:17825256[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Komatsu M, Chiba T, Tatsumi K, Iemura S, Tanida I, Okazaki N, Ueno T, Kominami E, Natsume T, Tanaka K. A novel protein-conjugating system for Ufm1, a ubiquitin-fold modifier. EMBO J. 2004 May 5;23(9):1977-86. Epub 2004 Apr 8. PMID:15071506 doi:http://dx.doi.org/10.1038/sj.emboj.7600205
- ↑ Mizushima T, Tatsumi K, Ozaki Y, Kawakami T, Suzuki A, Ogasahara K, Komatsu M, Kominami E, Tanaka K, Yamane T. Crystal structure of Ufc1, the Ufm1-conjugating enzyme. Biochem Biophys Res Commun. 2007 Nov 3;362(4):1079-84. Epub 2007 Aug 30. PMID:17825256 doi:10.1016/j.bbrc.2007.08.129
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