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| | ==Crystal structue of the cytosolic domain of T. maritima FeoB iron iransporter in GDP form II== | | ==Crystal structue of the cytosolic domain of T. maritima FeoB iron iransporter in GDP form II== |
| - | <StructureSection load='3a1t' size='340' side='right' caption='[[3a1t]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='3a1t' size='340' side='right'caption='[[3a1t]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3a1t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A1T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A1T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3a1t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A1T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A1T FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ais|3ais]], [[3aiu|3aiu]], [[3aiv|3aiv]], [[3aiw|3aiw]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TM0051 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a1t OCA], [https://pdbe.org/3a1t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a1t RCSB], [https://www.ebi.ac.uk/pdbsum/3a1t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a1t ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a1t OCA], [http://pdbe.org/3a1t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3a1t RCSB], [http://www.ebi.ac.uk/pdbsum/3a1t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3a1t ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9WXQ8_THEMA Q9WXQ8_THEMA] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 43589]] | + | [[Category: Large Structures]] |
| - | [[Category: Hattori, M]] | + | [[Category: Thermotoga maritima]] |
| - | [[Category: Ishitani, R]] | + | [[Category: Hattori M]] |
| - | [[Category: Nureki, O]] | + | [[Category: Ishitani R]] |
| - | [[Category: Feob]] | + | [[Category: Nureki O]] |
| - | [[Category: G protein]]
| + | |
| - | [[Category: Gdi]]
| + | |
| - | [[Category: Iron transpoter]]
| + | |
| - | [[Category: Small gtpase]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
Q9WXQ8_THEMA
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The FeoB family proteins are widely distributed prokaryotic membrane proteins involved in Fe(2+) uptake. FeoB consists of N-terminal cytosolic and C-terminal transmembrane domains. The N-terminal region of the cytosolic domain is homologous to small GTPase (G) proteins and is considered to regulate Fe(2+) uptake. The spacer region connecting the G and TM domains reportedly functions as a GDP dissociation inhibitor (GDI)-like domain that stabilizes the GDP-binding state. However, the function of the G and GDI-like domains in iron uptake remains unclear. Here, we report the structural and functional analyses of the FeoB cytosolic domain from Thermotoga maritima. The structure-based mutational analysis indicated that the interaction between the G and GDI-like domains is important for both the GDI and Fe(2+) uptake activities. On the basis of these results, we propose a regulatory mechanism of Fe(2+) uptake.
Structural basis of novel interactions between the small-GTPase and GDI-like domains in prokaryotic FeoB iron transporter.,Hattori M, Jin Y, Nishimasu H, Tanaka Y, Mochizuki M, Uchiumi T, Ishitani R, Ito K, Nureki O Structure. 2009 Oct 14;17(10):1345-55. Epub 2009 Sep 3. PMID:19733088[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hattori M, Jin Y, Nishimasu H, Tanaka Y, Mochizuki M, Uchiumi T, Ishitani R, Ito K, Nureki O. Structural basis of novel interactions between the small-GTPase and GDI-like domains in prokaryotic FeoB iron transporter. Structure. 2009 Oct 14;17(10):1345-55. Epub 2009 Sep 3. PMID:19733088 doi:10.1016/j.str.2009.08.007
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