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| | ==Crystal Structure of Monomeric Kusabira-Orange (MKO), Orange-Emitting GFP-like Protein, at pH 6.0== | | ==Crystal Structure of Monomeric Kusabira-Orange (MKO), Orange-Emitting GFP-like Protein, at pH 6.0== |
| - | <StructureSection load='2zmw' size='340' side='right' caption='[[2zmw]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='2zmw' size='340' side='right'caption='[[2zmw]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2zmw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Funco Funco]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ejp 2ejp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZMW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZMW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2zmw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lithophyllon_concinna Lithophyllon concinna]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2ejp 2ejp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZMW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZMW FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CFY:[(4Z)-2-{(2R)-2-[(1S)-1-AMINO-2-PHENYLETHYL]-2-HYDROXY-2,5-DIHYDRO-1,3-THIAZOL-4-YL}-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>CFY</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2zmu|2zmu]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CFY:[(4Z)-2-{(2R)-2-[(1S)-1-AMINO-2-PHENYLETHYL]-2-HYDROXY-2,5-DIHYDRO-1,3-THIAZOL-4-YL}-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>CFY</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mKO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=496660 FUNCO])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zmw OCA], [https://pdbe.org/2zmw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zmw RCSB], [https://www.ebi.ac.uk/pdbsum/2zmw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zmw ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zmw OCA], [http://pdbe.org/2zmw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zmw RCSB], [http://www.ebi.ac.uk/pdbsum/2zmw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zmw ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q6I7B2_LITCO Q6I7B2_LITCO] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Funco]] | + | [[Category: Large Structures]] |
| - | [[Category: Fukumura, E]] | + | [[Category: Lithophyllon concinna]] |
| - | [[Category: Karasawa, S]] | + | [[Category: Fukumura E]] |
| - | [[Category: Kikuchi, A]] | + | [[Category: Karasawa S]] |
| - | [[Category: Miyawaki, A]] | + | [[Category: Kikuchi A]] |
| - | [[Category: Mizuno, H]] | + | [[Category: Miyawaki A]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Mizuno H]] |
| - | [[Category: Shiro, Y]] | + | [[Category: Shiro Y]] |
| - | [[Category: Gfp-like protein]]
| + | |
| - | [[Category: Luminescent protein]]
| + | |
| - | [[Category: National project on protein structural and functional analyse]]
| + | |
| - | [[Category: Nppsfa]]
| + | |
| - | [[Category: Rsgi]]
| + | |
| Structural highlights
Function
Q6I7B2_LITCO
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Monomeric Kusabira Orange (mKO) is a green fluorescent protein (GFP)-like protein that emits orange light at a peak of 559 nm. We analyzed its X-ray structure at 1.65 A and found a novel three-ring chromophore that developed autocatalytically from a Cys65-Tyr66-Glu67 tripeptide in which the side chain of Cys65 formed the third 2-hydroxy-3-thiazoline ring. As a result, the chromophore contained the CNCOH group at the 2-position of the imidazolinone moiety such that the conjugated pi-electron system of the chromophore was more extended than that of GFP but less extended than that of the Discosoma sp. red fluorescent protein (DsRed). Since a sulfur atom has potent nucleophilic character, the third 3-thiazoline ring is rapidly and completely cyclized. Furthermore, our structure reveals the presence of a pi-pi stacking interaction between His197 and the chromophore as well as a pi-cation interaction between Arg69 and the chromophore. These structural findings are sufficient to account for the orange emission, pH tolerance, and photostability of mKO.
Structural Characterization of a Thiazoline-Containing Chromophore in an Orange Fluorescent Protein, Monomeric Kusabira Orange.,Kikuchi A, Fukumura E, Karasawa S, Mizuno H, Miyawaki A, Shiro Y Biochemistry. 2008 Oct 10. PMID:18844376[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kikuchi A, Fukumura E, Karasawa S, Mizuno H, Miyawaki A, Shiro Y. Structural Characterization of a Thiazoline-Containing Chromophore in an Orange Fluorescent Protein, Monomeric Kusabira Orange. Biochemistry. 2008 Oct 10. PMID:18844376 doi:10.1021/bi800727v
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