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| | ==Crystal structure of Aristaless and Clawless homeodomains bound to DNA== | | ==Crystal structure of Aristaless and Clawless homeodomains bound to DNA== |
| - | <StructureSection load='3a01' size='340' side='right' caption='[[3a01]], [[Resolution|resolution]] 2.70Å' scene=''> | + | <StructureSection load='3a01' size='340' side='right'caption='[[3a01]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3a01]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A01 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A01 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3a01]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A01 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3a02|3a02]], [[3a03|3a03]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">C15, CG7937 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME]), al, CG3935 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a01 OCA], [https://pdbe.org/3a01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a01 RCSB], [https://www.ebi.ac.uk/pdbsum/3a01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a01 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a01 OCA], [http://pdbe.org/3a01 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3a01 RCSB], [http://www.ebi.ac.uk/pdbsum/3a01 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3a01 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/AL_DROME AL_DROME]] Involved in the morphogenesis of proximal and distal pattern elements in a subset of appendages. Also has a role in early imaginal disk development. | + | [https://www.uniprot.org/uniprot/Q9VD99_DROME Q9VD99_DROME] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| - | [[Category: Kojima, T]] | + | [[Category: Large Structures]] |
| - | [[Category: Miyazono, K]] | + | [[Category: Kojima T]] |
| - | [[Category: Nagata, K]] | + | [[Category: Miyazono K]] |
| - | [[Category: Saigo, K]] | + | [[Category: Nagata K]] |
| - | [[Category: Tanokura, M]] | + | [[Category: Saigo K]] |
| - | [[Category: Developmental protein]]
| + | [[Category: Tanokura M]] |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Gene regulation-dna complex]]
| + | |
| - | [[Category: Homeobox]]
| + | |
| - | [[Category: Homeodomain]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Protein-dna complex]]
| + | |
| Structural highlights
Function
Q9VD99_DROME
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
To achieve accurate gene regulation, some homeodomain proteins bind cooperatively to DNA to increase those site specificities. We report a ternary complex structure containing two homeodomain proteins, aristaless (Al) and clawless (Cll), bound to DNA. Our results show that the extended conserved sequences of the Cll homeodomain are indispensable to cooperative DNA binding. In the Al-Cll-DNA complex structure, the residues in the extended regions are used not only for the intermolecular contacts between the two homeodomain proteins but also for the sequence-recognition mechanism of DNA by direct interactions. The residues in the extended N-terminal arm lie within the minor groove of DNA to form direct interactions with bases, whereas the extended conserved region of the C-terminus of the homeodomain interacts with Al to stabilize and localize the third alpha helix of the Cll homeodomain. This structure suggests a novel mode for the cooperativity of homeodomain proteins.
Cooperative DNA-binding and sequence-recognition mechanism of aristaless and clawless.,Miyazono K, Zhi Y, Takamura Y, Nagata K, Saigo K, Kojima T, Tanokura M EMBO J. 2010 May 5;29(9):1613-23. Epub 2010 Apr 13. PMID:20389279[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Miyazono K, Zhi Y, Takamura Y, Nagata K, Saigo K, Kojima T, Tanokura M. Cooperative DNA-binding and sequence-recognition mechanism of aristaless and clawless. EMBO J. 2010 May 5;29(9):1613-23. Epub 2010 Apr 13. PMID:20389279 doi:10.1038/emboj.2010.53
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