Insertase
From Proteopedia
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| - | + | <StructureSection load='4utq' size='340' side='right' caption='Cryo EM structure of E. coli insertase (cyan) complex with membrane domain of ATP synthase (green) PDB code [[4utq]].' scene='79/799601/Cv/1'> | |
| - | <StructureSection load='4utq' size='340' side='right' caption='Cryo EM structure of E. coli insertase ( | + | |
'''Insertase''' (Ins) catalyzes the insertion of proteins into the prokaryotic plasma membrane. Ins interacts with its substrate in a groove-like structure at an ampiphilic protein-lipid interface allowing the transmembrane segments of the substrate to slide into the lipid bilayer<ref>PMID:27879020</ref>. | '''Insertase''' (Ins) catalyzes the insertion of proteins into the prokaryotic plasma membrane. Ins interacts with its substrate in a groove-like structure at an ampiphilic protein-lipid interface allowing the transmembrane segments of the substrate to slide into the lipid bilayer<ref>PMID:27879020</ref>. | ||
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| - | == Function == | ||
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| - | == Disease == | ||
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| - | == Relevance == | ||
== Structural highlights == | == Structural highlights == | ||
| - | + | E. Coli Ins (YidC) contains 6 transmembrane segments. <scene name='79/799601/Cv/2'>Two of these segments was found to interact with a helical segments of a protein which is a candidate for insertion into a plasma membrane</scene><ref>PMID:18996118</ref>. | |
</StructureSection> | </StructureSection> | ||
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[[5m5h]], [[3wvf]] - EcIns YidC - ''Escherichia coli'' - Cryo EM<br /> | [[5m5h]], [[3wvf]] - EcIns YidC - ''Escherichia coli'' - Cryo EM<br /> | ||
| + | [[6al2]] - EcIns YidC <br /> | ||
[[3bs6]] - EcIns YidC periplasmic domain<br /> | [[3bs6]] - EcIns YidC periplasmic domain<br /> | ||
[[4utq]] - EcIns YidC + ATP synthase subunit C - Cryo EM<br /> | [[4utq]] - EcIns YidC + ATP synthase subunit C - Cryo EM<br /> | ||
| + | [[5mg3]] - EcIns YidC + SECY + SECE + SECG + SECD + SECF - Cryo EM<br /> | ||
[[3wo7]], [[3wo6]] - Ins YidC - ''Bacillus halodurans''<br /> | [[3wo7]], [[3wo6]] - Ins YidC - ''Bacillus halodurans''<br /> | ||
[[4c4v]] - EcIns BamA reasidues 344-808<br /> | [[4c4v]] - EcIns BamA reasidues 344-808<br /> | ||
| + | [[6fsu]] - EcIns BamA reasidues 421-810<br /> | ||
[[5ekq]] - Ins BamA + BamC + BamD + BamE <br /> | [[5ekq]] - Ins BamA + BamC + BamD + BamE <br /> | ||
[[4k3b]] - Ins BamA - ''Neisseria gonorrhoeae'' <br /> | [[4k3b]] - Ins BamA - ''Neisseria gonorrhoeae'' <br /> | ||
[[4k3c]] - Ins BamA - ''Haemophilus Ducreyi''<br /> | [[4k3c]] - Ins BamA - ''Haemophilus Ducreyi''<br /> | ||
| - | [[5y82]], [[5y83]] - | + | [[5y82]], [[5y83]] - TmIns YidC (mutant) - ''Thermotoga maritima''<br /> |
| + | [[6y86]] - TmIns YidC <br /> | ||
== References == | == References == | ||
<references/> | <references/> | ||
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| + | [[Category:Topic Page]] | ||
Current revision
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3D structures of insertase
Updated on 03-October-2021
5m5h, 3wvf - EcIns YidC - Escherichia coli - Cryo EM
6al2 - EcIns YidC
3bs6 - EcIns YidC periplasmic domain
4utq - EcIns YidC + ATP synthase subunit C - Cryo EM
5mg3 - EcIns YidC + SECY + SECE + SECG + SECD + SECF - Cryo EM
3wo7, 3wo6 - Ins YidC - Bacillus halodurans
4c4v - EcIns BamA reasidues 344-808
6fsu - EcIns BamA reasidues 421-810
5ekq - Ins BamA + BamC + BamD + BamE
4k3b - Ins BamA - Neisseria gonorrhoeae
4k3c - Ins BamA - Haemophilus Ducreyi
5y82, 5y83 - TmIns YidC (mutant) - Thermotoga maritima
6y86 - TmIns YidC
References
- ↑ Kuhn A, Kiefer D. Membrane protein insertase YidC in bacteria and archaea. Mol Microbiol. 2017 Feb;103(4):590-594. doi: 10.1111/mmi.13586. Epub 2017 Jan 3. PMID:27879020 doi:http://dx.doi.org/10.1111/mmi.13586
- ↑ Klenner C, Yuan J, Dalbey RE, Kuhn A. The Pf3 coat protein contacts TM1 and TM3 of YidC during membrane biogenesis. FEBS Lett. 2008 Dec 10;582(29):3967-72. doi: 10.1016/j.febslet.2008.10.044. Epub , 2008 Nov 6. PMID:18996118 doi:http://dx.doi.org/10.1016/j.febslet.2008.10.044
