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| | ==Solution NMR structure for B. pseudomallei BPSL1050== | | ==Solution NMR structure for B. pseudomallei BPSL1050== |
| - | <StructureSection load='2mpe' size='340' side='right' caption='[[2mpe]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='2mpe' size='340' side='right'caption='[[2mpe]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mpe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MPE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MPE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mpe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_K96243 Burkholderia pseudomallei K96243]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MPE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MPE FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BPSL1050 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272560 BURPS])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mpe OCA], [http://pdbe.org/2mpe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mpe RCSB], [http://www.ebi.ac.uk/pdbsum/2mpe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mpe ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mpe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mpe OCA], [https://pdbe.org/2mpe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mpe RCSB], [https://www.ebi.ac.uk/pdbsum/2mpe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mpe ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q63W39_BURPS Q63W39_BURPS] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Burps]] | + | [[Category: Burkholderia pseudomallei K96243]] |
| - | [[Category: Gaudesi, D]] | + | [[Category: Large Structures]] |
| - | [[Category: Musco, G]] | + | [[Category: Gaudesi D]] |
| - | [[Category: Quilici, G]] | + | [[Category: Musco G]] |
| - | [[Category: Melioidosis]] | + | [[Category: Quilici G]] |
| - | [[Category: Unknown function]]
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| Structural highlights
Function
Q63W39_BURPS
Publication Abstract from PubMed
Burkholderia pseudomallei is the etiological agent of melioidosis, a severe endemic disease in South-East Asia, causing septicemia and organ failure with high mortality rates. Current treatments and diagnostic approaches are largely ineffective. The development of new diagnostic tools and vaccines toward effective therapeutic opportunities against B. pseudomallei is therefore an urgent priority. In the framework of a multidisciplinary project tackling melioidosis through reverse and structural vaccinology, BPSL1050 was identified as a candidate for immunodiagnostic and vaccine development based on its reactivity against the sera of melioidosis patients. We determined its NMR solution structure and dynamics, and by novel computational methods we predicted immunogenic epitopes that once synthesized were able to elicit the production of antibodies inducing the agglutination of the bacterium and recognizing both BPSL1050 and B. pseudomallei crude extracts. Overall, these results hold promise for novel chemical biology approaches in the discovery of new diagnostic and prophylactic tools against melioidosis.
Structure-Based Design of a B Cell Antigen from B. pseudomallei.,Gaudesi D, Peri C, Quilici G, Gori A, Ferrer-Navarro M, Conchillo-Sole O, Thomas R, Nithichanon A, Lertmemongkolchai G, Titball R, Daura X, Colombo G, Musco G ACS Chem Biol. 2014 Dec 22. PMID:25495888[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gaudesi D, Peri C, Quilici G, Gori A, Ferrer-Navarro M, Conchillo-Sole O, Thomas R, Nithichanon A, Lertmemongkolchai G, Titball R, Daura X, Colombo G, Musco G. Structure-Based Design of a B Cell Antigen from B. pseudomallei. ACS Chem Biol. 2014 Dec 22. PMID:25495888 doi:http://dx.doi.org/10.1021/cb500831y
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