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| | ==Remarkable improvement of the heat stability of CutA1 from E.coli by rational protein designing== | | ==Remarkable improvement of the heat stability of CutA1 from E.coli by rational protein designing== |
| - | <StructureSection load='3ah6' size='340' side='right' caption='[[3ah6]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='3ah6' size='340' side='right'caption='[[3ah6]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3ah6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AH6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AH6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3ah6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AH6 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3aa8|3aa8]], [[3aa9|3aa9]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cutA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ah6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ah6 OCA], [https://pdbe.org/3ah6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ah6 RCSB], [https://www.ebi.ac.uk/pdbsum/3ah6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ah6 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ah6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ah6 OCA], [http://pdbe.org/3ah6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ah6 RCSB], [http://www.ebi.ac.uk/pdbsum/3ah6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ah6 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CUTA_ECOLI CUTA_ECOLI]] Involved in resistance toward heavy metals.<ref>PMID:7623666</ref> | + | [https://www.uniprot.org/uniprot/CUTA_ECOLI CUTA_ECOLI] Involved in resistance toward heavy metals.<ref>PMID:7623666</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[CutA1|CutA1]] | + | *[[CutA1 3D structures|CutA1 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| - | [[Category: Bagautdinov, B]] | + | [[Category: Large Structures]] |
| - | [[Category: Kunishima, N]] | + | [[Category: Bagautdinov B]] |
| - | [[Category: Matsuura, Y]] | + | [[Category: Kunishima N]] |
| - | [[Category: Tanaka, T]] | + | [[Category: Matsuura Y]] |
| - | [[Category: Yutani, K]] | + | [[Category: Tanaka T]] |
| - | [[Category: Copper tolerance]]
| + | [[Category: Yutani K]] |
| - | [[Category: Cuta1]]
| + | |
| - | [[Category: Point mutation]]
| + | |
| - | [[Category: Stability]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| Structural highlights
Function
CUTA_ECOLI Involved in resistance toward heavy metals.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
To enhance the heat stability of the CutA1 protein from Escherichia coli (EcCutA1) so that it has comparable stability to CutA1 from Pyrococcus horikoshii with a denaturation temperature (T(d)) of 150( degrees )C, we used the Stability Profile of Mutant Protein (SPMP) to examine the structure-sequence (3D-1D) compatibility between the conformation of EcCutA1 and its native sequence (J. Mol. Biol., 248, 733-738, (1995)). We identified seven residues in EcCutA1 that were incompatible in terms of dihedral angles and hydrophobicity. These residues were replaced with appropriate amino acids, and the mutant proteins were evaluated for changes in stability by DSC and denaturant denaturation. The mutations that were introduced at five out of the seven positions improved the stability of EcCutA1. The T(d) values of single (S11A) and triple (S11V/E61V/Q73V) mutants improved by 16.5 and 26.6( degrees )C, respectively, compared to that of the wild-type protein (89.9( degrees )C). These analyses showed that (1) the stability of EcCutA1 is remarkably improved by slight substitutions, even though the stability of the wild-type protein is considerably high, (2) remarkable improvements in the stability can be quantitatively explained based on the newly solved native structure, and (3) SPMP is a powerful tool to examine substitutions that improve protein stability.
Remarkable improvement in the heat stability of CutA1 from Escherichia coli by rational protein design.,Matsuura Y, Ota M, Tanaka T, Takehira M, Ogasahara K, Bagautdinov B, Kunishima N, Yutani K J Biochem. 2010 Jul 16. PMID:20639520[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Fong ST, Camakaris J, Lee BT. Molecular genetics of a chromosomal locus involved in copper tolerance in Escherichia coli K-12. Mol Microbiol. 1995 Mar;15(6):1127-37. PMID:7623666
- ↑ Matsuura Y, Ota M, Tanaka T, Takehira M, Ogasahara K, Bagautdinov B, Kunishima N, Yutani K. Remarkable improvement in the heat stability of CutA1 from Escherichia coli by rational protein design. J Biochem. 2010 Jul 16. PMID:20639520 doi:10.1093/jb/mvq079
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