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| - | [[Image:2tob.gif|left|200px]] | |
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| - | {{Structure
| + | ==SOLUTION STRUCTURE OF THE TOBRAMYCIN-RNA APTAMER COMPLEX, NMR, 13 STRUCTURES== |
| - | |PDB= 2tob |SIZE=350|CAPTION= <scene name='initialview01'>2tob</scene>
| + | <StructureSection load='2tob' size='340' side='right'caption='[[2tob]]' scene=''> |
| - | |SITE=
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=2TB:1,3-DIAMINO-4,5,6-TRIHYDROXY-CYCLOHEXANE'>2TB</scene>, <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=TOA:3-DEOXY-3-AMINO+GLUCOSE'>TOA</scene>, <scene name='pdbligand=TOC:2,3,6-TRIDEOXY-2,6-DIAMINO+GLUCOSE'>TOC</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| + | <table><tr><td colspan='2'>[[2tob]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TOB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2TOB FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2TB:1,3-DIAMINO-4,5,6-TRIHYDROXY-CYCLOHEXANE'>2TB</scene>, <scene name='pdbligand=TOA:3-DEOXY-3-AMINO+GLUCOSE'>TOA</scene>, <scene name='pdbligand=TOC:2,3,6-TRIDEOXY-2,6-DIAMINO+GLUCOSE'>TOC</scene></td></tr> |
| - | |DOMAIN=
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2tob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2tob OCA], [https://pdbe.org/2tob PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2tob RCSB], [https://www.ebi.ac.uk/pdbsum/2tob PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2tob ProSAT]</span></td></tr> |
| - | |RELATEDENTRY=
| + | </table> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2tob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2tob OCA], [http://www.ebi.ac.uk/pdbsum/2tob PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2tob RCSB]</span>
| + | __TOC__ |
| - | }}
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | '''SOLUTION STRUCTURE OF THE TOBRAMYCIN-RNA APTAMER COMPLEX, NMR, 13 STRUCTURES'''
| + | [[Category: Synthetic construct]] |
| - | | + | [[Category: Jiang L]] |
| - | | + | [[Category: Patel DJ]] |
| - | ==Overview==
| + | |
| - | We have solved the solution structure of the aminoglycoside antibiotic tobramycin complexed with a stem-loop RNA aptamer. The 14 base loop of the RNA aptamer 'zippers up' alongside the attached stem through alignment of four mismatches and one Watson-Crick pair on complex formation. The tobramycin inserts into the deep groove centered about the mismatch pairs and is partially encapsulated between its floor and a looped out guanine base that flaps over the bound antibiotic. Several potential intermolecular hydrogen bonds between the charged NH3 groups of tobramycin and acceptor atoms on base pair edges and backbone phosphates anchor the aminoglycoside antibiotic within its sequence/structure specific RNA binding pocket.
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| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2TOB is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2TOB OCA].
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| - | | + | |
| - | ==Reference==
| + | |
| - | Solution structure of the tobramycin-RNA aptamer complex., Jiang L, Patel DJ, Nat Struct Biol. 1998 Sep;5(9):769-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9731769 9731769]
| + | |
| - | [[Category: Protein complex]]
| + | |
| - | [[Category: Jiang, L.]] | + | |
| - | [[Category: Patel, D J.]] | + | |
| - | [[Category: aminoglycoside-rna recognition]] | + | |
| - | [[Category: tobramycin]] | + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:03:54 2008''
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