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| | ==Crystal structure of the single-stranded DNA binding protein from Mycobacterium leprae (Form II)== | | ==Crystal structure of the single-stranded DNA binding protein from Mycobacterium leprae (Form II)== |
| - | <StructureSection load='3afq' size='340' side='right' caption='[[3afq]], [[Resolution|resolution]] 2.80Å' scene=''> | + | <StructureSection load='3afq' size='340' side='right'caption='[[3afq]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3afq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycle Mycle]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AFQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AFQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3afq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_leprae_TN Mycobacterium leprae TN]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AFQ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3afp|3afp]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ssb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272631 MYCLE])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3afq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3afq OCA], [https://pdbe.org/3afq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3afq RCSB], [https://www.ebi.ac.uk/pdbsum/3afq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3afq ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3afq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3afq OCA], [http://pdbe.org/3afq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3afq RCSB], [http://www.ebi.ac.uk/pdbsum/3afq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3afq ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SSB_MYCLE SSB_MYCLE] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| - | *[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]] | + | *[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Mycle]] | + | [[Category: Large Structures]] |
| - | [[Category: Kaushal, P S]] | + | [[Category: Mycobacterium leprae TN]] |
| - | [[Category: Muniyappa, K]] | + | [[Category: Kaushal PS]] |
| - | [[Category: Sharma, A]] | + | [[Category: Muniyappa K]] |
| - | [[Category: Singh, P]] | + | [[Category: Sharma A]] |
| - | [[Category: Vijayan, M]] | + | [[Category: Singh P]] |
| - | [[Category: Changes on oligomerisation]]
| + | [[Category: Vijayan M]] |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Dna damage]]
| + | |
| - | [[Category: Dna repair]]
| + | |
| - | [[Category: Dna replication]]
| + | |
| - | [[Category: Dna-binding]]
| + | |
| - | [[Category: Ob-fold]]
| + | |
| - | [[Category: Quaternary structure and stability]]
| + | |
| - | [[Category: Water-bridge]]
| + | |
| Structural highlights
Function
SSB_MYCLE
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The crystal structures of two forms of Mycobacterium leprae single-stranded DNA-binding protein (SSB) have been determined at 2.05 and 2.8 A resolution. Comparison of these structures with the structures of other eubacterial SSBs indicates considerable variation in their quaternary association, although the DNA-binding domains in all of them exhibit the same OB-fold. This variation has no linear correlation with sequence variation, but could be related to variation in protein stability. Molecular-dynamics simulations have been carried out on tetrameric molecules derived from the two forms and the prototype Escherichia coli SSB and the individual subunits of both proteins. Together, the X-ray studies and molecular-dynamics simulations yield information on the relatively rigid and flexible regions of the molecule and on the effect of oligomerization on flexibility. The simulations provide insight into the changes in subunit structure on oligomerization. They also provide insight into the stability and time evolution of the hydrogen bonds/water bridges that connect the two pairs of monomers in the tetramer.
X-ray and molecular-dynamics studies on Mycobacterium leprae single-stranded DNA-binding protein and comparison with other eubacterial SSB structures.,Kaushal PS, Singh P, Sharma A, Muniyappa K, Vijayan M Acta Crystallogr D Biol Crystallogr. 2010 Oct;66(Pt 10):1048-58. Epub 2010 Sep, 18. PMID:20944238[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kaushal PS, Singh P, Sharma A, Muniyappa K, Vijayan M. X-ray and molecular-dynamics studies on Mycobacterium leprae single-stranded DNA-binding protein and comparison with other eubacterial SSB structures. Acta Crystallogr D Biol Crystallogr. 2010 Oct;66(Pt 10):1048-58. Epub 2010 Sep, 18. PMID:20944238 doi:10.1107/S0907444910032208
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