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6hyp
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6hyp is ON HOLD until sometime in the future Authors: Sosnowski, P., Urnavicius, L., Boland, A., Fagiewicz, R., Busselez, J., Papai, G., Schmidt, H....) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Rea1 Wild type ADP state (AAA+ ring part)== | |
| + | <SX load='6hyp' size='340' side='right' viewer='molstar' caption='[[6hyp]], [[Resolution|resolution]] 4.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6hyp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HYP FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.4Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hyp OCA], [https://pdbe.org/6hyp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hyp RCSB], [https://www.ebi.ac.uk/pdbsum/6hyp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hyp ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MDN1_YEAST MDN1_YEAST] Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits. Functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus. Mediates ATP-dependent remodeling of the pre-ribosome just prior to export to allow maturation of 60S subunits into export-competent particles. Removes the ribosome biogenesis factor RSA4 from pre-60S ribosomal subunits in the nucleoplasm to drive nuclear export of the subunit. Involved in 3' processing of the 5.8S rRNA.<ref>PMID:15260980</ref> <ref>PMID:15528184</ref> <ref>PMID:19737519</ref> <ref>PMID:20542003</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The biogenesis of 60S ribosomal subunits is initiated in the nucleus where rRNAs and proteins form pre-60S particles. These pre-60S particles mature by transiently interacting with various assembly factors. The ~5000 amino-acid AAA+ ATPase Rea1 (or Midasin) generates force to mechanically remove assembly factors from pre-60S particles, which promotes their export to the cytosol. Here we present three Rea1 cryoEM structures. We visualise the Rea1 engine, a hexameric ring of AAA+ domains, and identify an alpha-helical bundle of AAA2 as a major ATPase activity regulator. The alpha-helical bundle interferes with nucleotide-induced conformational changes that create a docking site for the substrate binding MIDAS domain on the AAA +ring. Furthermore, we reveal the architecture of the Rea1 linker, which is involved in force generation and extends from the AAA+ ring. The data presented here provide insights into the mechanism of one of the most complex ribosome maturation factors. | ||
| - | + | The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1.,Sosnowski P, Urnavicius L, Boland A, Fagiewicz R, Busselez J, Papai G, Schmidt H Elife. 2018 Nov 26;7. pii: 39163. doi: 10.7554/eLife.39163. PMID:30460895<ref>PMID:30460895</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6hyp" style="background-color:#fffaf0;"></div> |
| - | [[Category: Boland | + | == References == |
| - | [[Category: Busselez | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </SX> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Sosnowski | + | [[Category: Saccharomyces cerevisiae]] |
| + | [[Category: Boland A]] | ||
| + | [[Category: Busselez J]] | ||
| + | [[Category: Fagiewicz R]] | ||
| + | [[Category: Papai G]] | ||
| + | [[Category: Schmidt H]] | ||
| + | [[Category: Sosnowski P]] | ||
| + | [[Category: Urnavicius L]] | ||
Current revision
Rea1 Wild type ADP state (AAA+ ring part)
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