This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
6mui
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6mui is ON HOLD Authors: Hasan, S.S., Sun, C., Kim, A.S., Watanabe, Y., Chen, C.L., Klose, T., Buda, G., Crispin, M., Diamond, M.S., Klimstra, W.B.,...) |
|||
| (6 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==CryoEM structure of chimeric Eastern Equine Encephalitis Virus with Fab of EEEV-42 antibody== | |
| + | <SX load='6mui' size='340' side='right' viewer='molstar' caption='[[6mui]], [[Resolution|resolution]] 7.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6mui]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Eastern_equine_encephalitis_virus Eastern equine encephalitis virus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6MUI FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6mui FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mui OCA], [http://pdbe.org/6mui PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mui RCSB], [http://www.ebi.ac.uk/pdbsum/6mui PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mui ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Alphaviruses are enveloped pathogens that cause arthritis and encephalitis. Here, we report a 4.4-A cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans. Our analysis provides insights into viral entry into host cells. The envelope protein E2 showed a binding site for the cellular attachment factor heparan sulfate. The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system. A mechanism for nucleocapsid core release and disassembly upon viral entry was inferred based on pH changes and capsid dissociation from envelope proteins. The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release. Using five Fab-EEEV complexes derived from neutralizing antibodies, our investigation provides insights into EEEV host cell interactions and protective epitopes relevant to vaccine design. | ||
| - | + | Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.,Hasan SS, Sun C, Kim AS, Watanabe Y, Chen CL, Klose T, Buda G, Crispin M, Diamond MS, Klimstra WB, Rossmann MG Cell Rep. 2018 Dec 11;25(11):3136-3147.e5. doi: 10.1016/j.celrep.2018.11.067. PMID:30540945<ref>PMID:30540945</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6mui" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| + | *[[Antibody 3D structures|Antibody 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Eastern equine encephalitis virus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Buda, G]] | ||
| + | [[Category: Chen, C L]] | ||
[[Category: Crispin, M]] | [[Category: Crispin, M]] | ||
| - | [[Category: | + | [[Category: Diamond, M S]] |
| - | [[Category: | + | [[Category: Hasan, S S]] |
| - | [[Category: | + | [[Category: Kim, A S]] |
| - | [[Category: | + | [[Category: Klimstra, W B]] |
[[Category: Klose, T]] | [[Category: Klose, T]] | ||
| - | [[Category: | + | [[Category: Rossmann, M G]] |
| - | [[Category: | + | [[Category: Sun, C]] |
| + | [[Category: Watanabe, Y]] | ||
| + | [[Category: Alphavirus]] | ||
| + | [[Category: Eeev]] | ||
| + | [[Category: Fab]] | ||
| + | [[Category: Sindbi]] | ||
| + | [[Category: Virus-immune system complex]] | ||
Current revision
CryoEM structure of chimeric Eastern Equine Encephalitis Virus with Fab of EEEV-42 antibody
| |||||||||
