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6egk

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==T181N Cucumene Synthase==
==T181N Cucumene Synthase==
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<StructureSection load='6egk' size='340' side='right' caption='[[6egk]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
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<StructureSection load='6egk' size='340' side='right'caption='[[6egk]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6egk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EGK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EGK FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6egk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EGK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EGK FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6egk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6egk OCA], [http://pdbe.org/6egk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6egk RCSB], [http://www.ebi.ac.uk/pdbsum/6egk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6egk ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6egk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6egk OCA], [https://pdbe.org/6egk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6egk RCSB], [https://www.ebi.ac.uk/pdbsum/6egk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6egk ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/D5SLU6_STRCL D5SLU6_STRCL]
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Linear triquinanes are sesquiterpene natural products with hydrocarbon skeletons consisting of three fused five-membered rings. Importantly, several of these compounds exhibit useful anticancer, anti-inflammatory, and antibiotic properties. However, linear triquinanes pose significant challenges to organic synthesis because of the structural and stereochemical complexity of their hydrocarbon skeletons. To illuminate nature's solution to the generation of linear triquinanes, we now describe the crystal structure of Streptomyces clavuligerus cucumene synthase. This sesquiterpene cyclase catalyzes the stereospecific cyclization of farnesyl diphosphate to form a linear triquinane product, (5 S,7 S,10 R,11 S)-cucumene. Specifically, we report the structure of the wild-type enzyme at 3.05 A resolution and the structure of the T181N variant at 1.96 A resolution, both in the open active site conformations without any bound ligands. The high-resolution structure of T181N cucumene synthase enables inspection of the active site contour, which adopts a three-dimensional shape complementary to a linear triquinane. Several aromatic residues outline the active site contour and are believed to facilitate cation-pi interactions that would stabilize carbocation intermediates in catalysis. Thus, aromatic residues in the active site not only define the template for catalysis but also play a role in reducing activation barriers in the multistep cyclization cascade.
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Crystal Structure of Cucumene Synthase, a Terpenoid Cyclase That Generates a Linear Triquinane Sesquiterpene.,Blank PN, Pemberton TA, Chow JY, Poulter CD, Christianson DW Biochemistry. 2018 Oct 22. doi: 10.1021/acs.biochem.8b00899. PMID:30346736<ref>PMID:30346736</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6egk" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Blank, P N]]
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[[Category: Large Structures]]
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[[Category: Christianson, D W]]
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[[Category: Streptomyces clavuligerus]]
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[[Category: Pemberton, T A]]
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[[Category: Blank PN]]
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[[Category: Lyase]]
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[[Category: Christianson DW]]
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[[Category: Open conformation]]
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[[Category: Pemberton TA]]
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[[Category: Terpene cyclase]]
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[[Category: Triquinane scaffold]]
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T181N Cucumene Synthase

PDB ID 6egk

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