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| | ==Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri== | | ==Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri== |
| - | <StructureSection load='6gyb' size='340' side='right' caption='[[6gyb]], [[Resolution|resolution]] 3.28Å' scene=''> | + | <SX load='6gyb' size='340' side='right' viewer='molstar' caption='[[6gyb]], [[Resolution|resolution]] 3.28Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6gyb]] is a 42 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanac Xanac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GYB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GYB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6gyb]] is a 42 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_citri_pv._citri_str._306 Xanthomonas citri pv. citri str. 306]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GYB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GYB FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">XAC2622 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=190486 XANAC]), virB9, XAC2620 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=190486 XANAC]), virB10, XAC2619 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=190486 XANAC])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.28Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gyb OCA], [http://pdbe.org/6gyb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gyb RCSB], [http://www.ebi.ac.uk/pdbsum/6gyb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gyb ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gyb OCA], [https://pdbe.org/6gyb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gyb RCSB], [https://www.ebi.ac.uk/pdbsum/6gyb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gyb ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| - | [[Category: Xanac]] | + | [[Category: Large Structures]] |
| - | [[Category: Costa, T R.D]] | + | [[Category: Xanthomonas citri pv. citri str. 306]] |
| - | [[Category: Farah, C S]] | + | [[Category: Costa TRD]] |
| - | [[Category: Sgro, G G]] | + | [[Category: Farah CS]] |
| - | [[Category: Waksman, G]] | + | [[Category: Sgro GG]] |
| - | [[Category: Bacterial killing]] | + | [[Category: Waksman G]] |
| - | [[Category: Bacterial type iv secretion system]]
| + | |
| - | [[Category: Core complex]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Type IV secretion (T4S) systems form the most common and versatile class of secretion systems in bacteria, capable of injecting both proteins and DNAs into host cells. T4S systems are typically composed of 12 components that form 2 major assemblies: the inner membrane complex embedded in the inner membrane and the core complex embedded in both the inner and outer membranes. Here we present the 3.3 A-resolution cryo-electron microscopy model of the T4S system core complex from Xanthomonas citri, a phytopathogen that utilizes this system to kill bacterial competitors. An extensive mutational investigation was performed to probe the vast network of protein-protein interactions in this 1.13-MDa assembly. This structure expands our knowledge of the molecular details of T4S system organization, assembly and evolution.
Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri.,Sgro GG, Costa TRD, Cenens W, Souza DP, Cassago A, Coutinho de Oliveira L, Salinas RK, Portugal RV, Farah CS, Waksman G Nat Microbiol. 2018 Oct 22. pii: 10.1038/s41564-018-0262-z. doi:, 10.1038/s41564-018-0262-z. PMID:30349081[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sgro GG, Costa TRD, Cenens W, Souza DP, Cassago A, Coutinho de Oliveira L, Salinas RK, Portugal RV, Farah CS, Waksman G. Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri. Nat Microbiol. 2018 Oct 22. pii: 10.1038/s41564-018-0262-z. doi:, 10.1038/s41564-018-0262-z. PMID:30349081 doi:http://dx.doi.org/10.1038/s41564-018-0262-z
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